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  • Interaction between calpain-1 and HSP90: new insights into the regulation of localization and activity of the protease.

Interaction between calpain-1 and HSP90: new insights into the regulation of localization and activity of the protease.

PloS one (2015-01-13)
Monica Averna, Roberta De Tullio, Marco Pedrazzi, Margherita Bavestrello, Matteo Pellegrini, Franca Salamino, Sandro Pontremoli, Edon Melloni
ZUSAMMENFASSUNG

Here we demonstrate that heat shock protein 90 (HSP90) interacts with calpain-1, but not with calpain-2, and forms a discrete complex in which the protease maintains its catalytic activity, although with a lower affinity for Ca2+. Equilibrium gel distribution experiments show that this complex is composed by an equal number of molecules of each protein partner. Moreover, in resting cells, cytosolic calpain-1 is completely associated with HSP90. Since calpain-1, in association with HSP90, retains its proteolytic activity, and the chaperone is displaced by calpastatin also in the absence of Ca2+, the catalytic cleft of the protease is not involved in this association. Thus, calpain-1 can form two distinct complexes depending on the availability of calpastatin in the cytosol. The occurrence of a complex between HSP90 and calpain-1, in which the protease is still activable, can prevent the complete inhibition of the protease even in the presence of high calpastatin levels. We also demonstrate that in basal cell conditions HSP90 and calpain-1, but not calpain-2, are inserted in the multi-protein N-Methyl-D-Aspartate receptor (NMDAR) complex. The amount of calpain-1 at the NMDAR cluster is not modified in conditions of increased [Ca2+]i, and this resident protease is involved in the processing of NMDAR components. Finally, the amount of calpain-1 associated with NMDAR cluster is independent from Ca2+-mediated translocation. Our findings show that HSP90 plays an important role in maintaining a given and proper amount of calpain-1 at the functional sites.

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Produktbeschreibung

Sigma-Aldrich
Monoclonal Anti-Calpastatin antibody produced in mouse, clone 1F7E3D10, ascites fluid, buffered aqueous solution
Sigma-Aldrich
Monoclonal Anti-μ-Calpain, Large Subunit antibody produced in mouse, clone 15C10, purified immunoglobulin
Sigma-Aldrich
Monoclonal Anti-m-Calpain (Domain III/IV) antibody produced in mouse, clone 107-82, ascites fluid