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  • The glycan shield of HIV is predominantly oligomannose independently of production system or viral clade.

The glycan shield of HIV is predominantly oligomannose independently of production system or viral clade.

PloS one (2011-08-23)
Camille Bonomelli, Katie J Doores, D Cameron Dunlop, Victoria Thaney, Raymond A Dwek, Dennis R Burton, Max Crispin, Christopher N Scanlan
ZUSAMMENFASSUNG

The N-linked oligomannose glycans of HIV gp120 are a target for both microbicide and vaccine design. The extent of cross-clade conservation of HIV oligomannose glycans is therefore a critical consideration for the development of HIV prophylaxes. We measured the oligomannose content of virion-associated gp120 from primary virus from PBMCs for a range of viral isolates and showed cross-clade elevation (62-79%) of these glycans relative to recombinant, monomeric gp120 (∼30%). We also confirmed that pseudoviral production systems can give rise to notably elevated gp120 oligomannose levels (∼98%), compared to gp120 derived from a single-plasmid viral system using the HIV(LAI) backbone (56%). This study highlights differences in glycosylation between virion-associated and recombinant gp120.