Direkt zum Inhalt
Merck
  • Nuclear proteasomes carry a constitutive posttranslational modification which derails SDS-PAGE (but not CTAB-PAGE).

Nuclear proteasomes carry a constitutive posttranslational modification which derails SDS-PAGE (but not CTAB-PAGE).

Biochimica et biophysica acta (2014-09-07)
David S Pitcher, Kate de Mattos-Shipley, Ziming Wang, Konstantinos Tzortzis, Katerina Goudevenou, Helen Flynn, Georg Bohn, Amin Rahemtulla, Irene Roberts, Ambrosius P Snijders, Anastasios Karadimitris, Maurits F Kleijnen
ZUSAMMENFASSUNG

We report that subunits of human nuclear proteasomes carry a previously unrecognised, constitutive posttranslational modification. Subunits with this modification are not visualised by SDS-PAGE, which is used in almost all denaturing protein gel electrophoresis. In contrast, CTAB-PAGE readily visualises such modified subunits. Thus, under most experimental conditions, with identical samples, SDS-PAGE yielded gel electrophoresis patterns for subunits of nuclear proteasomes which were misleading and strikingly different from those obtained with CTAB-PAGE. Initial analysis indicates a novel modification of a high negative charge with some similarity to polyADP-ribose, possibly explaining compatibility with (positively-charged) CTAB-PAGE but not (negatively-charged) SDS-PAGE and providing a mechanism for how nuclear proteasomes may interact with chromatin, DNA and other nuclear components.

MATERIALIEN
Produktnummer
Marke
Produktbeschreibung

Millipore
HIS-Select® HF-Nickel-Affinitätsgel