ZRB2675

Anti-δ Tubulin Antibody, clone 1B20 ZooMAb^® Rabbit Monoclonal

recombinant, expressed in HEK 293 cells

• Synonym(e): Delta-tubulin, Tubulin delta chain
• UNSPSC-Code: 12352203
• NACRES: NA.43

Eigenschaften

• Biologische Quelle: rabbit
• Qualitätsniveau: 200
• Rekombinant: expressed in HEK 293 cells
• Konjugat: unconjugated
• Antikörperform: purified antibody
• Antikörper-Produkttyp: primary antibodies
• Klon: 1B20, recombinant monoclonal
• Beschreibung: recombinant, expressed in HEK 293 cells
• Produktlinie: ZooMAb^® learn more
• Form: lyophilized
• Mol-Gew.: calculated mol wt 51.03 kDa; observed mol wt ~53 kDa
• Aufgereinigt durch: using Protein A
• Speziesreaktivität: human, mouse, rat
• Verpackung: antibody small pack of 25
• Grünere Alternativprodukt-Eigenschaften: Waste Prevention; Designing Safer Chemicals; Design for Energy Efficiency; Learn more about the Principles of Green Chemistry.
• Erweiterte Validierung: recombinant expression; Learn more about Antibody Enhanced Validation
• Methode(n): affinity binding assay: suitable; immunocytochemistry: suitable; immunohistochemistry: suitable; western blot: suitable
• Isotyp: IgG
• Epitopsequenz: N-terminal half
• Protein-ID-Hinterlegungsnummer: NP_057345
• UniProt-Hinterlegungsnummer: Q9UJT1
• Grünere Alternativprodukt-Kategorie: Aligned
• Versandbedingung: ambient
• Lagertemp.: 2-8°C
• Angaben zum Gen: human ... TUBD1(51174)

Beschreibung

Allgemeine Beschreibung

We are committed to bringing you greener alternative products, which adhere to one or more of the 12 Principles of Green Chemistry. This antibody is preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed, and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.

ZooMAb^® antibodies represent an entirely new generation of recombinant monoclonal antibodies. Each ZooMAb^® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb^® antibodies are reliably available and ready to ship when you need them.

Spezifität

Clone 1B20 is a ZooMAb^® mouse recombinant monoclonal antibody that specifically detects Tubulin. It targets an epitope within 23 amino acids from the N-terminal half.

Immunogen

KLH-conjugated linear peptide corresponding to 23 amino acids from the N-terminal half of human Tubulin.

Anwendung

Quality Control Testing

Evaluated by Western Blotting in Mouse testis tissue lysate.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected Tubulin in Mouse testis tissue lysate.

Tested Applications

Western Blotting Analysis: A 1:10,000 dilution from a representative lot detected Tubulin in lysates from K562 cells and Rat testis tissue.

Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected Tubulin in HepG2 cells.

Affinity Binding Assay: A representative lot of this antibody bound Tubulin with a KD of 1.0 x 10-12 in an affinity binding assay.

Immunohistochemistry (Paraffin) Analysis: A 1:1,000 dilution from a representative lot detected Tubulin in Human stomach tissue sections.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

Zielbeschreibung

Tubulin delta chain (UniProt: Q9UJT1; also known as Delta-tubulin) is encoded by the TUBD1 (also known as TUBD) gene (Gene ID: 51174) in human. The importance of tubulins , β, and has been well recognized, however, the function and mechanism of the action of , , and tubulins is not well understood. Tubulin may regulate centriole function in a wide range of species. It also acts as a positive regulator of hedgehog signaling and regulates ciliary function. In unicellular species and mammalian cell lines, mutations in - Tubulin are reported to cause centriole destabilization and atypical mitosis and, in the most severe cases, cell death. - and -tubulin are reported to retain the /β/ -tubulin conserved N-terminal sequence in their nucleotide-binding domain, required for GTP-binding. This ZooMAb^® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Stathatos, GG., et al. (2021). Trends Cell Biol. 31(9); 774-787).

Physikalische Form

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 µL.

Rekonstituierung

300 µg/mL after reconstitution at 25 µL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Lagerung und Haltbarkeit

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 µL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

Rechtliche Hinweise

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

Haftungsausschluss

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Sicherheitshinweise

• Lagerklassenschlüssel: 11 - Combustible Solids
• WGK: WGK 1
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable