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R8507

Sigma-Aldrich

Hpa I from Haemophilus parainfluenzae

Restriction Enzyme

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About This Item

CAS-Nummer:
81295-24-1
EC-Nummer:
3.1.21.4 (BRENDA, IUBMB)
MDL-Nummer:
MFCD00166572
UNSPSC-Code:
12352204

Qualität

for molecular biology

Form

buffered aqueous glycerol solution

Konzentration

3,000-10,000 units/mL

Versandbedingung

wet ice

Lagertemp.

−20°C

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Spezifität

Recognition sequence: 5′-GTT/AAC-3′
Ligation and recutting results: After 2-10-fold Hpa I overdigestion of 1 μg λ DNA substrate, results in 100% cutting, >80% of fragments can be ligated, and >95% recut.
Heat inactivation: Not completely inactivated at 65 °C for 15 minutes.

Anwendung

HpaI is a restriction endonuclease that is used to cleave DNA at the recognition site 5′-GTT/AAC-3′, generating fragments with blunt ends.

Sonstige Hinweise

Supplied with 10x Restriction Enzyme Buffer SA (B7531).

Physikalische Form

Solution in 20 mM Tris-HCl, pH 7.5, 50 mM KCl, 0.5 mM EDTA, 5 mM 2-mercaptoethanol, 0.01% polydocanol, 50% glycerol (v/v) at 4°C

Inkubationspuffer

Produkt-Nr.
Beschreibung
Preisangaben

B7531

Restriction Enzyme Buffer SA, 10 ×

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Produkt-Nr.
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Lagerklassenschlüssel

12 - Non Combustible Liquids

WGK

WGK 1

Flammpunkt (°F)

Not applicable

Flammpunkt (°C)

Not applicable

Analysenzertifikate (COA)

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L Q Gu et al.
The Journal of general physiology, 118(5), 481-494 (2001-11-07)
Noncovalent molecular adapters, such as cyclodextrins, act as binding sites for channel blockers when lodged in the lumen of the alpha-hemolysin (alphaHL) pore, thereby offering a basis for the detection of a variety of organic molecules with alphaHL as a
Tatiana Gianni et al.
Journal of virology, 78(22), 12268-12276 (2004-10-28)
Herpes simplex virus (HSV) enters cells by fusion with target membranes, commonly the plasma membrane. In some cells, including CHO cells expressing the nectin1 or herpesvirus entry mediator receptors, entry occurs through an endocytic route. We report the following results.
Nucleotide sequences at the cleavage sites of two restriction endonucleases from Hemophilus parainfluenzae.
D E Garfin et al.
Biochemical and biophysical research communications, 59(1), 108-116 (1974-07-10)
Jaroslav Jelinek et al.
Epigenetics, 7(12), 1368-1378 (2012-10-19)
Genome wide analysis of DNA methylation provides important information in a variety of diseases, including cancer. Here, we describe a simple method, Digital Restriction Enzyme Analysis of Methylation (DREAM), based on next generation sequencing analysis of methylation-specific signatures created by
Saqib H Ansari et al.
Journal of pediatric hematology/oncology, 35(4), e153-e156 (2013-02-08)
β-thalassemia is characterized by impaired β-chain synthesis leading to ineffective erythropoiesis, severe anemia, and a need for blood transfusion. Presence of Xmn I polymorphism (-158 C-T nucleotide change) in γ-globin gene is associated with a higher fetal hemoglobin and a
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