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P6855

Sigma-Aldrich

Protein A–Gold

~2.5 A520 units/mL, aqueous glycerol suspension, 17-23 nm (monodisperse), 20 nm nominal (colloidal gold)

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About This Item

MDL-Nummer:
MFCD00132107
UNSPSC-Code:
12352203

Konjugat

gold conjugate

Form

aqueous glycerol suspension

Konzentration

~2.5 A520 units/mL

Partikelgröße

17-23 nm (monodisperse)

Nominal

20 nm (colloidal gold)

Lagertemp.

2-8°C

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Allgemeine Beschreibung

Protein A is a 42 kD, highly stable cell surface receptor isolated from Staphylococcus aureus which binds selectively and with high affinity to the Fc portion of IgG from various species.Protein A-gold is an electron dense, non-fading marker and can be used as a probe in electron microscopy (TEM and SEM), light microscopy and blotting. Protein A - 20 nm colloidal gold reacts specifically with IgG of human.

Anwendung

Protein A-Gold can be used in dot blot. It can also be used to detect immunoglobulins.

Sonstige Hinweise

Protein A, extracellular (P 6031), adsorbed to colloidal gold.

Physikalische Form

Suspension in 0.02 M Tris-buffered saline, containing 1% bovine serum albumin, 30% glycerol, and 15 mM sodium azide.

Haftungsausschluss

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Lagerklassenschlüssel

10 - Combustible liquids

WGK

WGK 3

Flammpunkt (°F)

Not applicable

Flammpunkt (°C)

Not applicable

Analysenzertifikate (COA)

Suchen Sie nach Analysenzertifikate (COA), indem Sie die Lot-/Chargennummer des Produkts eingeben. Lot- und Chargennummern sind auf dem Produktetikett hinter den Wörtern ‘Lot’ oder ‘Batch’ (Lot oder Charge) zu finden.

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Some physiochemical properties of protein A from Staphylococcus aureus.
I Björk et al.
European journal of biochemistry, 29(3), 579-584 (1972-09-25)
Bacterial Fc receptors.
Boyle, M.D.P., and Reis, K.J.
Biotechnology, 5, 697-703 (1987)
Letizia Penolazzi et al.
Frontiers in cell and developmental biology, 11, 1180774-1180774 (2023-05-22)
Identifying the subcellular localization of a protein within a cell is often an essential step in understanding its function. The main objective of this report was to determine the presence of the P2X7 receptor (P2X7R) in healthy human cells of
Regina Rabl et al.
The Journal of cell biology, 185(6), 1047-1063 (2009-06-17)
Crista junctions (CJs) are important for mitochondrial organization and function, but the molecular basis of their formation and architecture is obscure. We have identified and characterized a mitochondrial membrane protein in yeast, Fcj1 (formation of CJ protein 1), which is
Lipid droplets and peroxisomes are co-regulated to drive lifespan extension in response to mono-unsaturated fatty acids.
Papsdorf, et al.
Nature Cell Biology, 25, 672-684 (2023)
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