CRISPR15H
Lenti CRISPR Non-Target gRNA Control Transduction Particles, High Titer (LV02 vector)
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About This Item
Empfohlene Produkte
Form
liquid
Qualitätsniveau
Verpackung
vial of 8 x 25 μL
Konzentration
(5x108 TU/ml (via p24 titering assay))
Anwendung(en)
CRISPR
Versandbedingung
dry ice
Lagertemp.
−70°C
Allgemeine Beschreibung
The Lenti CRISPR Non-Target gRNA Control Transduction Particles (LV02 vector) include a gRNA sequence that does not target known human, mouse and rat genes. It is useful as a negative control in experiments using CRISPR lentiviral clones. The lenti CRISPR non-target control uses a dual-component system consisting of U6-driven guide RNA (non-target) and Ef1a-driven Puromycin resistance cassette. This control requires Cas9 be delivered by a separate vector.
Lentiviral-based particles permit efficient infection and integration of the construct into differentiated and non-dividing cells, such as neurons and dendritic cells, overcoming low transfection and integration difficulties when using these cell lines. Self-inactivating replication incompetent viral particles are produced in packaging cells (HEK293T) by co-transfection with compatible packaging and envelope plasmids.
Lentiviral-based particles permit efficient infection and integration of the construct into differentiated and non-dividing cells, such as neurons and dendritic cells, overcoming low transfection and integration difficulties when using these cell lines. Self-inactivating replication incompetent viral particles are produced in packaging cells (HEK293T) by co-transfection with compatible packaging and envelope plasmids.
Anwendung
Functional Genomics/Screening/Target Validation
Sonstige Hinweise
Ampicillin and puromycin antibiotic resistance genes provide selection in bacterial or mammalian cells, respectively.
Rechtliche Hinweise
Lagerklassenschlüssel
12 - Non Combustible Liquids
WGK
WGK 3
Flammpunkt (°F)
Not applicable
Flammpunkt (°C)
Not applicable
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