K-BALB (K-234)

89092101, mouse embryo, Fibroblast

• UNSPSC-Code: 41106514

Eigenschaften

• product name: K-BALB (K-234), 89092101
• Biologische Quelle: mouse embryo
• Wachstumsmodus: Adherent
• Karyotyp: 2n=40
• Morphologie: Fibroblast
• Produkte: Not specified
• Rezeptoren: Not specified
• Methode(n): cell culture | mammalian: suitable
• Versandbedingung: dry ice
• Lagertemp.: −196°C

Beschreibung

Ursprung der Zelllinie

Mouse A31 embryo Kirsten sarcoma, transformed

Beschreibung der Zelllinie

Cell morphology is different from the parent clone (A31) and there is a loss of sensitivity to contact inhibition of cell division. The cells are highly tumorigenic. Superinfection with MuLV results in rescue of MSV.

Nährmedium

DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Subkultur-Routine

Split sub-confluent cultures (70-80%) 1:4 to 1:10 i.e. seeding at 1-3 x 10,000 cells/cm² using 0.1% trypsin for 3-5 minutes; 5% CO₂; 37°C

Sonstige Hinweise

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