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01696

Sigma-Aldrich

Acrylamid

for Northern and Southern blotting, powder blend

Synonym(e):

2-Propenamid, Acrylsäureamid

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About This Item

Lineare Formel:
CH2=CHCONH2
CAS-Nummer:
79-06-1
Molekulargewicht:
71.08
Beilstein:
605349
EG-Nummer:
201-173-7
MDL-Nummer:
MFCD00008032
UNSPSC-Code:
41105319
eCl@ss:
39031205
PubChem Substanz-ID:
329747346
NACRES:
NA.25

Qualität

for molecular biology

Qualitätsniveau

200

Dampfdichte

2.45 (vs air)

Dampfdruck

0.03 mmHg ( 40 °C)

Produktlinie

BioReagent

Assay

≥99.5% (GC)

Form

powder blend

Methode(n)

electrophoresis: suitable

Verunreinigungen

DNases, none detected
 RNases, none detected
 phosphatases, none detected
 proteases, none detected

bp

125 °C/25 mmHg (lit.)

mp (Schmelzpunkt)

82-86 °C (lit.)

Löslichkeit

H2O: 0.25 g/mL at 20 °C, clear, colorless

Lagertemp.

2-8°C

SMILES String

NC(=O)C=C

InChI

1S/C3H5NO/c1-2-3(4)5/h2H,1H2,(H2,4,5)

InChIKey

HRPVXLWXLXDGHG-UHFFFAOYSA-N

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Allgemeine Beschreibung

Acrylamide powder is a white, odorless, crystalline solid. It is commonly used in molecular biology labs as a cross linking agent to cast polyacrylamide gels for nucleic acid electrophoresis.

Anwendung

Suitable for making polyacrylamide gels according to established recipes.

Vorsicht

Acrylamide powder is highly toxic through inhalation and skin contact. Acrylamide is considered to be a probable carciongen in humans. Standard laboratory nitrile gloves and full sleeve lab coats should be worn when handling acrylamide in any form. Dust masks or working in a fume hood is advised when handling acrylamide powder.

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Piktogramme

Skull and crossbonesHealth hazard
GHS06,GHS08

Signalwort

Danger

Gefahreneinstufungen

Acute Tox. 3 Oral - Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Carc. 1B - Eye Irrit. 2 - Muta. 1B - Repr. 2 - Skin Irrit. 2 - Skin Sens. 1 - STOT RE 1 Oral

Zielorgane

Peripheral nervous system

Lagerklassenschlüssel

6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

WGK

WGK 3

Flammpunkt (°F)

280.4 °F - closed cup

Flammpunkt (°C)

138 °C - closed cup

Persönliche Schutzausrüstung

Eyeshields, Faceshields, Gloves, type P3 (EN 143) respirator cartridges

Choose from one of the most recent versions:

Analysenzertifikate (COA)

Lot/Batch Number
BCCK3074
BCCH8174
BCCH0715
BCCJ5814
BCCG2561

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If you require a particular version, you can look up a specific certificate by the Lot or Batch number.

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Die Dokumentenbibliothek aufrufen

R.C. Ogden et al.
Methods in Enzymology, 152, 66-66 (1987)
Janneke G F Hogervorst et al.
Carcinogenesis, 35(5), 1032-1038 (2014-01-09)
Acrylamide, a probable human carcinogen, is present in heat-treated carbohydrate-rich foods. Epidemiological studies have not shown a clear association between acrylamide intake and colorectal cancer (CRC) risk. This may be due to the molecular heterogeneity in colorectal tumors, which was
Rusty L Montgomery et al.
EMBO molecular medicine, 6(10), 1347-1356 (2014-09-23)
Over the last decade, great enthusiasm has evolved for microRNA (miRNA) therapeutics. Part of the excitement stems from the fact that a miRNA often regulates numerous related mRNAs. As such, modulation of a single miRNA allows for parallel regulation of
Franco Pedreschi et al.
Journal of the science of food and agriculture, 94(1), 9-20 (2013-08-14)
Acrylamide (AA) is known as a neurotoxin in humans and it is classified as a probable human carcinogen by the International Agency of Research on Cancer. AA is produced as by-product of the Maillard reaction in starchy foods processed at
Arianna Rath et al.
Proceedings of the National Academy of Sciences of the United States of America, 110(39), 15668-15673 (2013-09-11)
SDS/PAGE is universally used in biochemistry, cell biology, and immunology to resolve minute protein amounts readily from tissue and cell extracts. Although molecular weights of water-soluble proteins are reliably determined from their SDS/PAGE mobility, most helical membrane proteins, which comprise
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Protokolle

Northern & Southern Blot Protocols

Learn Northern and Southern blotting basics, with protocols and applications for macromolecule transfer to membrane supports.

Northern und Southern Blot Protokolle und Einführung

Einführung in Northern Blotting und Southern Blotting, bei denen es sich um beliebte Methoden zur Übertragung von Makromolekülen auf Membranen handelt. Dieser Artikel enthält außerdem ein Protokoll für Northern Blotting und ein Protokoll für Southern Blotting.

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