QIA88
ProteoExtract® Cytosol/Mitochondria Fractionation Kit
Synonym(e):
Cellular Fractionation Kit
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About This Item
UNSPSC-Code:
41106500
Empfohlene Produkte
Verwendung
sufficient for 100 extractions
Hersteller/Markenname
Calbiochem®
Lagerbedingungen
OK to freeze
avoid repeated freeze/thaw cycles
Methode(n)
DNA extraction: suitable
RNA extraction: suitable
nuclear extraction: suitable
protein extraction: suitable
Aufnahme
sample type cells
Versandbedingung
dry ice
Lagertemp.
−20°C
Allgemeine Beschreibung
Note: 1 Test = reagents sufficient for processing 5 x 107 cells.
This kit provides a unique combination of reagents useful for the isolation of a highly enriched mitochondrial fraction from the cytosol. The enriched fractions can be used to study factors of interest using Western blotting, ELISA, or other assays.
Komponenten
Mitochondria Extraction Buffer, Cytosol Extraction Buffer, Protease Inhibitor Cocktail, Reducing Reagent, and a user protocol.
Warnhinweis
Toxicity: Multiple Toxicity Values, refer to MSDS (O)
Prinzip
The ProteoExtract Cytosol/Mitochondria Fractionation Kit is designed for extraction of mammalian cells to yield enriched mitochondrial and cytosolic fractions for use in downstream analyses, such as Western blotting.
Angaben zur Herstellung
• Protease Inhibitor Cocktail: Reconstitute the lyophilized Protease Inhibitor Cocktail by adding 250 µl DMSO; mix well before use. Dispense into aliquots and store at -20°C.• 1X Cytosol Extraction Buffer Mix: Prepare 1X Cytosol Extraction Buffer by diluting the Cytosol Extraction Buffer 1:4 with ddH2O. Prepare only the amount needed for the assay (5 x 107 cells require 1 ml 1X Cytosol Extraction Buffer Mix). Just prior to use add 2 µl Protease Inhibitor Cocktail and 1 µl DTT to 1 ml 1X Cytosol Extraction Buffer.• Mitochondria Extraction Buffer Mix: Prepare only the amount needed for the assay (5 x 107 pelleted cells require 100 µl Mitochondria Extraction Buffer Mix). Just prior to use, add 2 µl Protease Inhibitor Cocktail and 1 µl DTT to 1 ml of Mitochondria Extraction Buffer.
Lagerung und Haltbarkeit
Upon arrival store the entire contents of the kit at -20°C. Following initial use, store the buffers at 4°C and the DTT and Protease Inhibitor Cocktail at -20°C.
Sonstige Hinweise
Due to the nature of the Hazardous Materials in this shipment, additional shipping charges may be applied to your order. Certain sizes may be exempt from the additional hazardous materials shipping charges. Please contact your local sales office for more information regarding these charges.
Rechtliche Hinweise
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
PROTEOEXTRACT is a registered trademark of Merck KGaA, Darmstadt, Germany
Signalwort
Danger
H-Sätze
Gefahreneinstufungen
Acute Tox. 4 Oral - Aquatic Chronic 3 - Eye Dam. 1 - Skin Corr. 1B
Lagerklassenschlüssel
8A - Combustible corrosive hazardous materials
Analysenzertifikate (COA)
Suchen Sie nach Analysenzertifikate (COA), indem Sie die Lot-/Chargennummer des Produkts eingeben. Lot- und Chargennummern sind auf dem Produktetikett hinter den Wörtern ‘Lot’ oder ‘Batch’ (Lot oder Charge) zu finden.
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