CB1024

Anti-ACTN4 Mouse mAb (4D10)

liquid, clone 4D10, Calbiochem^®

• Synonym(e): Anti-Actinin α4
• UNSPSC-Code: 12352203
• NACRES: NA.41

Eigenschaften

• Biologische Quelle: mouse
• Qualitätsniveau: 100
• Antikörperform: purified antibody
• Antikörper-Produkttyp: primary antibodies
• Klon: 4D10, monoclonal
• Form: liquid
• Enthält nicht: preservative
• Speziesreaktivität: mouse, human, rat
• Hersteller/Markenname: Calbiochem^®
• Lagerbedingungen: OK to freeze; avoid repeated freeze/thaw cycles
• Isotyp: IgG2a
• Versandbedingung: wet ice
• Lagertemp.: −20°C
• Posttranslationale Modifikation Target: unmodified
• Angaben zum Gen: human ... ACTN4(81)

Beschreibung

Allgemeine Beschreibung

Anti-ACTN4, mouse monoclonal, clone 4D10, recognizes the ~90-100 kDa ACTN4 in PC-12, HeLa, Raw 264.7, MCF-7, NIH3T3 cells & in human testes. It is validated for ELISA, WB, ICC, and paraffin sections.

Purified mouse monoclonal antibody. Recognizes the ~90-100 kDa ACTN4 protein.

Recognizes the ~90-100 kDa ACTN4 protein in PC-12, HeLa, Raw 264.7, MCF-7, and NIH3T3 cells and human testes tissue.

Immunogen

A recombinant polypeptide corresponding to amino acids 592-702 of human ACTN4, espressed as a GST fusion protein

Anwendung

ELISA (see coments)
Immunoblotting (1-5 g/ml)
Immunocytochemistry (20 g/ml)
Paraffin Sections (3 g/ml)

Warnhinweis

Toxicity: Regulatory Review (Z)

Physikalische Form

In PBS, pH 7.2.

Rekonstituierung

Following initial thaw, aliquot and freeze (-20°C).

Hinweis zur Analyse

Negative Control
293T cells

Positive Control
PC-12 cells, HeLa cells, Raw 264.7 cells, MCF-7 cells, NIH3T3 cells, Human testes tissue

Sonstige Hinweise

For ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.

Yamada, S., et al. 2010. Int. J. Oral Maxillofac. Surg.39, 61.

Rechtliche Hinweise

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Sicherheitshinweise

• Lagerklassenschlüssel: 12 - Non Combustible Liquids
• WGK: nwg
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable