371207

Dkk-1, Mouse, Recombinant

Dkk-1, Mouse, Recombinant, consists of amino acids 30-272 and expressed in Chinese Hamster Ovary cells. Inhibits Wnt-3a-induced alkaline phosphatase production by MC3T3-E1 mouse osteoblastic cells.

• UNSPSC-Code: 12352203
• NACRES: NA.41

Eigenschaften

• Rekombinant: expressed in CHO cells
• Qualitätsniveau: 100
• Assay: ≥95% (SDS-PAGE)
• Form: liquid
• Hersteller/Markenname: Calbiochem^®
• Lagerbedingungen: OK to freeze
• Verunreinigungen: <1.0 EU/μg protein Endotoxin
• Versandbedingung: ambient
• Lagertemp.: −20°C

Beschreibung

Allgemeine Beschreibung

Recombinant, mouse Dickkopf related protein 1 (Dkk-1) consisting of amino acids 30-272 and expressed in Chinese Hamster Ovary cells. Inhibits Wnt-3a-induced alkaline phosphatase production by MC3T3-E1 mouse osteoblastic cells. ND₅₀ = 0.02-0.1 µg/ml.

Biochem./physiol. Wirkung

ND₅₀ = 0.02-0.1 µg/ml, in the presence of 10 ng/ml of rmWnt-3a, as measured by its ability to inhibit Wnt-3a-inducedalkaline phosphatase production by MC3T3-E1 mouse osteoblastic cells.

Warnhinweis

Toxicity: Standard Handling (A)

Physikalische Form

Lyophilized from 0.2 µm sterile-filtered PBS, BSA.

Rekonstituierung

Following reconstitution refrigerate (4°C) for short-term storage or aliquot and freeze (-20°C) for long-term storage. Stock solutions are stable for up to 1 month at 4°C or for up to 3 months at -20°C.

Reconstitute to 100 µg/ml in PBS containing at least 0.1% human or bovine serum albumin.

Sonstige Hinweise

Pinzone, J.J., et al. 2009. Blood113, 517.
Li, J., et al. 2006. Bone36, 754.
Morvan, F., et al. 2006. J. Bone Miner. Res.21, 934.
Niehrs, C., 2006. Oncogene25, 7469.
Verani, R., et al. 2006. J. Neurochem.101, 242.
Cappuccio, I., et al. 2005. J. Neurosci.25, 2647.
Kemp, C., et al. 2005. Dev. Dyn.233, 1064.
Adamska, M., et al. 2004. Dev. Biol.272, 134.
Bullock, C.M., et al. 2004. Mol. Pharmacol.65, 582.
Mao, B., et al. 2002. Nature417, 664.
Mao, B., et al. 2001. Nature411, 321.
Glinka, A., et al. 1998. Nature391, 357.

Rechtliche Hinweise

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Sicherheitshinweise

• Lagerklassenschlüssel: 11 - Combustible Solids
• WGK: WGK 3