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Merck

374210

Sigma-Aldrich

5-Brom-indoxyl-acetat

98%

Synonym(e):

3-Acetoxy-5-brom-indol, 3-Acetyl-5-brom-indoxyl

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About This Item

Empirische Formel (Hill-System):
C10H8BrNO2
CAS-Nummer:
Molekulargewicht:
254.08
Beilstein:
168698
EG-Nummer:
MDL-Nummer:
UNSPSC-Code:
12352100
PubChem Substanz-ID:
NACRES:
NA.22

Assay

98%

Form

powder

mp (Schmelzpunkt)

130-132 °C (lit.)

Löslichkeit

ethanol: soluble 50 mg/mL, clear, colorless

SMILES String

CC(=O)Oc1c[nH]c2ccc(Br)cc12

InChI

1S/C10H8BrNO2/c1-6(13)14-10-5-12-9-3-2-7(11)4-8(9)10/h2-5,12H,1H3

InChIKey

KFTGECHXNQBTNZ-UHFFFAOYSA-N

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Allgemeine Beschreibung

5-Bromoindoxyl acetate is a halogenated heterocyclic compound. It is widely used as esterase substrate.

Anwendung

5-Bromoindoxyl acetate may be used as substrate for the assay of insect esterases by employing DEAE (Diethylaminoethyl)-cellulose columns.
5-Bromoindoxyl acetate may be used:
  • as esterase substrate to investigate the activity of non-specific esterase in the matrix of developing bovine enamel
  • in histochemical studies of the nonspecific esterase of mouse epididymis
  • as substrate to investigate the esterase activity in guinea-pig thyroid and mouse epididymis epithelial cells
  • in staining procedure for the frozen sections of muscle fixed in buffered formaldehyde

Lagerklassenschlüssel

11 - Combustible Solids

WGK

WGK 3

Flammpunkt (°F)

Not applicable

Flammpunkt (°C)

Not applicable

Persönliche Schutzausrüstung

Eyeshields, Gloves, type N95 (US)


Analysenzertifikate (COA)

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Afsharpour F and O'Brien RD.
Journal of Insect Physiology, 9(4), 521-529 (1963)
S Kirkeby et al.
Archives of andrology, 6(2), 163-173 (1981-03-01)
Nonspecific esterase of mouse epididymis has previously been studied histochemically, using alpha naphthyl-acetate and 5-bromoindoxyl acetate techniques, as well as certain inhibitors. Epithelial cell types of the epididymis have been characterized, and certain esterase isozymes in a particular cell type
S Kirkeby et al.
Acta histochemica, 62(1), 44-56 (1978-01-01)
Esterase activity in guinea-pig thyroid and mouse epididymis epithelial cells has been studied using 5-bromoindoxyl acetate as substrate. The pattern of esterase activity in the thyroid of the guinea-pig is constant, irrespective of whether ferri-ferrocyanide (FFC) or certain copper compounds
Complete staining of neuromuscular innervation with bromoindigo and silver.
McIsaac G and Kiernan JA.
Biotechnic & Histochemistry, 49(4), 211-214 (1974)
S Kirkeby et al.
Acta histochemica, 83(1), 11-19 (1988-01-01)
Electrophoretic separation of non-specific esterases and esterproteases from kidney, lung, and liver have been carried out in polyacrylamide gels. By use of zone electrophoresis, isoelectric focusing, and 2-dimensional electrophoresis it was found that most of the esterprotease bands had the

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