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Merck

HPLC Analysis of Thyroglobulin, gamma-Globulins, Ovalbumin, Myoglobin, and Poly-DL-Alanine on Zenix® SEC-150, 30 cm x 7.8 mm I.D., 3 μm particles

HPLC Analysis of Thyroglobulin, gamma-Globulins, Ovalbumin, Myoglobin, and Poly-DL-Alanine on Zenix® SEC-150, 30 cm x 7.8 mm I.D., 3 μm particles application for HPLC

Materials

standard

Product No.
Description
Pricing

M1882

Myoglobin from equine heart

≥90% (SDS-PAGE), essentially salt-free, lyophilized powder

P9003

Poly-DL-alanine

mol wt 1,000-5,000

U0750

Uracil

≥99.0%

T1001

Thyroglobulin from bovine thyroid

powder, ≥90% (agarose gel electrophoresis)

A6075

Monoclonal Anti-Chicken Egg Albumin (Ovalbumin) antibody produced in mouse

clone OVA-14, ascites fluid

G5009

γ-Globulins from bovine blood

≥99% (agarose gel electrophoresis)

CONDITIONS

column

Zenix® SEC-150, 30 cm x 7.8 mm I.D., 3 μm particles (Z777018)

mobile phase

150 mM potassium phosphate monobasic, pH 7.0 (adjusted with potassium hydroxide)

flow rate

0.7 mL/min

pressure

890 psi (61.4 bar)

column temp.

25 °C

detector

UV, 214 nm

injection

1 μL

sample

1 g/L each (except uracil, 0.1 g/L ) in mobile phase A

Description

Analysis Note

This application demonstrates the suitability of Zenix SEC-150 for the separation of proteins. Separation is by inclusion into the pore volume with larger molecules being excluded and therefore eluting earlier.

Legal Information

Zenix is a registered trademark of Sepax Technologies

Chromatogram Search

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