HPLC Analysis of Histidine Enantiomers on Astec® CHIROBIOTIC® T (15 °C)
Materials
analytical column
Product No.
Description
Pricing
guard cartridge
Product No.
Description
Pricing
Astec® CHIROBIOTIC® T Chiral HPLC Guard Column
Cartridge, 5 μm particle size, L × I.D. 2 cm × 4 mmstandard
Product No.
Description
Pricing
L-Histidine
suitable for cell culture, meets EP, USP testing specifications, from non-animal sourceL-Histidine
certified reference material, TraceCERT®, Manufactured by: Sigma-Aldrich Production GmbH, SwitzerlandCONDITIONS
column
CHIROBIOTIC T, 25 cm x 4.6 mm I.D., 5 μm particles (12024AST)
mobile phase
[A] water; [B] methanol; [C] formic acid; (30:70:0.006, A:B:C)
flow rate
0.5 mL/min
column temp.
15 °C
detector
UV, 205 nm
injection
10 μL
sample
200 μg /mL in 50:50 water:methanol
Description
Legal Information
Astec is a registered trademark of Merck KGaA, Darmstadt, Germany
CHIROBIOTIC is a registered trademark of Sigma-Aldrich Co. LLC