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  • Comparative studies of HSV-1 antigens solubilised from infected cells by using non-ionic or zwitterionic detergents.

Comparative studies of HSV-1 antigens solubilised from infected cells by using non-ionic or zwitterionic detergents.

Journal of medical virology (1990-06-01)
R Jennings, M Erturk
ABSTRACT

HSV-1 antigen preparations solubilised from Vero cells by using either the non-ionic detergent Nonidet P40 or the zwitterionic detergent Empigen BB, and purified on sucrose density gradients or over a sucrose cushion, were tested by ELISA with anti-HSV-1 glycoprotein monoclonal antibodies and by radioimmunoprecipitation (RIP) with polyclonal HSV-1 antiserum. Amongst several proteins detected in these preparations, the four major HSV-1 glycoproteins, gB, gC, gD, and gE, were found to be present. Differences between NP40 or Empigen-solubilised HSV-1 antigen preparations with respect to two of these glycoproteins, gB and gE, were detected by using a small panel of monoclonal antibodies. Comparative studies in mice showed the Empigen-solubilised HSV-1 antigen preparations elicited greater antibody responses and greater protection against lethal HSV-1 challenge infection than the NP40-solubilised preparation.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
EMPIGEN® BB detergent, ~30% active substance