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C2888
Acetylcholinesterase from Electrophorus electricus (electric eel)
Type V-S, lyophilized powder, ≥1,000 units/mg protein
Synonym(s):
AChE, Acetylcholine acetylhydrolase, Cholinesterase, Acetyl
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| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| 500 units | Please contact Customer Service for Availability | CZK 5,200.00 | |
| 1000 units | Please contact Customer Service for Availability | CZK 9,890.00 | |
| 5000 units | Please contact Customer Service for Availability | CZK 22,900.00 |
About This Item
CAS Number:
UNSPSC Code:
12352204
NACRES:
NA.32
EC Number:
232-559-3
MDL number:
Specific activity:
≥1,000 units/mg protein
Biological source:
Electrophorus electricus
biological source
Electrophorus electricus
Quality Segment
type
Type V-S
form
lyophilized powder
specific activity
≥1,000 units/mg protein
mol wt
280 kDa
composition
Protein, ≥45% biuret
pI
~5.3
solubility
Tris buffer: 1 mg/mL (0.02 M Tris buffer, pH 7.5)
storage temp.
−20°C
General description
Molecular Weight: 280 kDa
Isoelectric Point: 5.5
Extinction Coefficient: E1% = 18.0 (280 nm)
Acetylcholinesterase from Electrophorus electricus is a tetramer composed of 4 equal subunits of 70 kDa each. Each subunit contains one active site. The enzyme is a glycoprotein containing hexosamines.
Isoelectric Point: 5.5
Extinction Coefficient: E1% = 18.0 (280 nm)
Acetylcholinesterase from Electrophorus electricus is a tetramer composed of 4 equal subunits of 70 kDa each. Each subunit contains one active site. The enzyme is a glycoprotein containing hexosamines.
Application
Biochem/physiol Actions
Acetylcholinesterase (AChE) exhibits its activity against acetylcholine in the neuromuscular junctions and synaptic cleft of cholinergic neurons.[2] It participates in the nitric oxide signaling pathway.[1]
Major degradative enzyme for acetylcholine in vivo. Converts acetylcholine + H2O to choline + acetic acid.
Physical form
Lyophilized powder containing Tris buffer salts
Analysis Note
The activity obtained using acetylcholine as substrate is 30-100 times that obtained with butyrylcholine, using acetylcholinesterase from electric eel.
Other Notes
One unit will hydrolyze 1.0 μmole of acetylcholine to choline and acetate per min at pH 8.0 at 37 °C.
Disclaimer
For dilute solutions (<1 mg/ml), add 1 mg/ml BSA to stabilize. Solutions containing at least 1 mg protein/ml in dilute neutral phosphate buffer are stable at least 6 months refrigerated.
1 of 1
This Item | |||
|---|---|---|---|
| biological source Electrophorus electricus | biological source - | biological source - | biological source - |
| specific activity ≥1,000 units/mg protein | specific activity 200-1,000 units/mg protein | specific activity ≥1,000 units/mg protein (BCA) | specific activity ≥10 units/mg protein |
| form lyophilized powder | form lyophilized powder | form lyophilized powder | form lyophilized powder |
| storage temp. −20°C | storage temp. −20°C | storage temp. −20°C | storage temp. −20°C |
| solubility Tris buffer: 1 mg/mL (0.02 M Tris buffer, pH 7.5) | solubility 20 mM Tris HCl buffer, pH 7.5: soluble 1.0 mg/mL, clear(lit.) | solubility - | solubility cold water: 60 U/mL |
| mol wt 280 kDa | mol wt 280 kDa | mol wt 64.6 kDa | mol wt tetramer 440 kDa |
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Storage Class
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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Related Content
Global Trade Item Number
| SKU | GTIN |
|---|---|
| C2888-5KU | 04061833492420 |
| C2888-500UN | 04061835543267 |
| C2888-1KU | 04061835555901 |
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What is the Department of Transportation shipping information for this product?
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Are solutions of Product C2888, Acetylcholinesterase from Electrophorus electricus (electric eel), harmed by freezing?
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Yes, solutions may be harmed by freezing. The data sheet for Product C2888 indicates that solutions stabilized by the addition of BSA are stable when kept refrigerated for up to six months. A customer has reported to us that they tried freezing a solution to which BSA was added; they found that the solution had a 40 percent loss of activity after four months in the freezer. So our best recommendation is that solutions be stabilized with BSA and kept refrigerated, not frozen.
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