MABE229

Anti-phospho-Cdk1 (Thr14, Tyr15)Antibody, clone CP3.2

clone CP3.2, from mouse

• Synonym(s): Cyclin-dependent kinase 1, CDK1, Cell division control protein 2 homolog, Cell division protein kinase 1, p34 protein kinase
• UNSPSC Code: 12352203
• NACRES: NA.41
• eCl@ss: 32160702
• Clone: CP3.2, monoclonal
• Species reactivity: human
• Application: ICC, WB
• Citations: 2

Properties

• biological source: mouse
• Quality Segment: 100
• antibody form: purified antibody
• antibody product type: primary antibodies
• clone: CP3.2, monoclonal
• species reactivity: human
• technique(s): immunocytochemistry: suitable, western blot: suitable
• isotype: IgG1κ
• NCBI accession no.: NP_001777
• UniProt accession no.: P06493
• shipped in: wet ice
• target post-translational modification: phosphorylation (pThr14, pTyr15)
• Gene Information: human ... CDK1(983)

Description

General description

Cyclin dependent kinase 1 (Cdk1) regulates cell cycle progression. It is active as a heterodimeric complex with cyclins. Cdk1 associates with cyclin A at the end of S phase and targets proteins such as p53, BARD1, and BRCA2 involved in cell cycle progression, and others involved in DNA replication. At the end of the G2 phase, Cdk1 associates with cyclin B1 and B2 to phosphorylate multiple protein targets required for transition from G2 to M phase, and progression through M phase. Cdk1 itself undergoes phosphorylation at Thr14 and Tyr15 by PKMYT1, which prevents nuclear translocation of Cdk1. Cdk1 is also phosphorylated at Tyr15 by WEE1/2 which inhibits both Cdk1 activity and transition from G2 to M phase. Conversely, dephosphorylation of Thr14 and Tyr15 by CDC25A/B activates Cdk1 and triggers mitosis. Cdk1 may also regulate epigenetic gene silencing through phosphorylation of EZH2 and maintenance of histone H3 lysine 27 trimethylation, which has been implicated in cancer.

~34 kDa observed. An uncharacterized band may be observed at ~62 kDa in some cell lysates.

Immunogen

Epitope: Phosphorylated Thr14 and phosphorylated Tyr15

Linear peptide corresponding to human Cdk1 phosphorylated at Thr14 and phosphorylated at Tyr15.

Application

Anti-phospho-Cdk1 (Thr14, Tyr15) Antibody, clone CP3.2 is a mouse monoclonal antibody for detection of phospho-Cdk1 (Thr14, Tyr15) also known as Cyclin-dependent kinase 1, or Cell division control protein 2 homolog & has been validated in WB, ICC.

Research Category
Epigenetics & Nuclear Function

Research Sub Category
Cell Cycle, DNA Replication & Repair

Biochem/physiol Actions

This antibody recognizes Cdk1 phosphorylated at Thr14 and phosphorylated at Tyr15.

Physical form

Format: Purified

Protein G Purified

Purified mouse monoclonal IgG1κ supernatant in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Preparation Note

Stable for 1 year at 2-8°C from date of receipt.

Analysis Note

Control
UV treated and untreated HeLa cell lysates

Evaluated by Western Blot in UV treated and untreated HeLa cell lysates.

Western Blot Analysis: A 1:1,000 dilution from a representative lot detected phospho-Cdk1 (Thr14, Tyr15) in UV treated HeLa cell lysates.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 12 - Non Combustible Liquids
• wgk: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable