MABC653

Anti-S100A4 Antibody, clone 9D5.1

clone 9D5.1, from mouse

• Synonym(s): Protein S100-A4, Calvasculin, Metastasin, Placental calcium-binding protein, Protein Mts1, S100 calcium-binding protein A4
• UNSPSC Code: 12352203
• NACRES: NA.41
• eCl@ss: 32160702
• Conjugate: unconjugated
• Clone: 9D5.1, monoclonal
• Application: ICC, WB
• Citations: -

Properties

• biological source: mouse
• Quality Segment: 100
• conjugate: unconjugated
• antibody form: purified immunoglobulin
• antibody product type: primary antibodies
• clone: 9D5.1, monoclonal
• species reactivity: human, mouse
• technique(s): immunocytochemistry: suitable, western blot: suitable
• isotype: IgG1κ
• NCBI accession no.: NP_002952
• UniProt accession no.: P26447
• target post-translational modification: unmodified
• Gene Information: human ... S100A4(6275) ; mouse ... S100A4(20198)

Description

General description

Protein S100-A4 (UniProt P26447; also known as Calvasculin, Metastasin, Placental calcium-binding protein, Protein Mts1, S100 calcium-binding protein A4) is encoded by the S100A4 (also known as CAPL, MTS1, P9KA, PEL98) gene (Gene ID 6275) in human. S100A4 belongs to a family of low moleclar weight proteins characterized by two calcium-binding helix-loop-helix EF-hand domains (a.a. 12-47 & 50-85 of human S100A4). S100 proteins exist as non-covalent homodimers with cell-specific expression patterns. S100A4 associates with cytoskeletal proteins, such as myosin and F-actin, as well as other actin-binding proteins such as Talin and integrins, and is involved in controlling not just shape and motility of normal cells, but also plays a role in cancer cells invasion/metastasis.

~12 kDa oberved. 11.73/11.72/11.78 kDa (human/mouse/rat) calculated. Uncharacterized band(s) may appear in some lysates.

Immunogen

Epitope: Within EF-hands 1&2.

GST-tagged recombinant human S100A4 internal fragment.

Application

Anti-S100A4 Antibody, clone 9D5.1 is an antibody against S100A4 for use in Western Blotting, Immunocytochemistry.

Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected S100A4 in HUVECs, HeLa, A431, and NIH/3T3 cells.

Research Category
Apoptosis & Cancer

Research Sub Category
Cytoskeletal Signaling

Biochem/physiol Actions

Clone 9D5.1 was raised against human S100-A4 internal fragment that shares high sequence homology with the corresponding region in mouse (96%) and rat (93%) protein.

Physical form

Format: Purified

Protein G Purified

Purified mouse monoclonal IgG1κ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Preparation Note

Stable for 1 year at 2-8°C from date of receipt.

Analysis Note

Evaluated by Western Blotting in HeLa cell lysate.

Western Blotting Analysis: 1.0 µg/mL of this antibody detected S100A4 in 10 µg of HeLa cell lysate.

Other Notes

Concentration: Please refer to lot specific datasheet.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 12 - Non Combustible Liquids
• wgk: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable