MAB5432

Anti-CREB Antibody

Chemicon^®, from mouse

• Synonym(s): cAMP-response Element Binding Protein
• UNSPSC Code: 12352203
• NACRES: NA.41
• eCl@ss: 32160702
• Conjugate: unconjugated
• Clone: monoclonal
• Application: ELISA, WB
• Citations: 13

Properties

• biological source: mouse
• Quality Segment: 100
• conjugate: unconjugated
• antibody form: purified immunoglobulin
• antibody product type: primary antibodies
• clone: monoclonal
• species reactivity: human, mouse, rat
• manufacturer/tradename: Chemicon^®
• technique(s): ELISA: suitable, western blot: suitable
• isotype: IgG1
• NCBI accession no.: NM_004379.2, NM_134442.2
• UniProt accession no.: P16220
• shipped in: wet ice
• target post-translational modification: unmodified
• Gene Information: human ... CREB1(1385)

Description

General description

CREB is a beta ZIP transcription factor that activates target genes through cAMP response elements. CREB is able to mediate signals from numerous physiological stimuli, resulting in regulation of a broad array of cellular responses. While CREB is expressed in numerous tissues, it plays a large regulatory role in the nervous system. CREB is believed to play a key role in promoting neuronal survival, precursor proliferation, neurite outgrowth and neuronal differentiation in certain neuronal populations. CREB promotes outgrowth and differentiation as a mediator of Neurotrophin pathways. CREB is able to selectively activate numerous downstream genes through interactions with different dimerization partners. CREB is activated by phosphorylation at Ser133 by various signaling pathways including Erk, Ca2+ and stress signaling.

Immunogen

Recombinant CREB-A protein.

Application

Anti-CREB Antibody is a Mouse Monoclonal Antibody for detection of CREB also known as cAMP-response Element Binding Protein & has been validated in ELISA & WB.

Research Category
Epigenetics & Nuclear Function

Research Sub Category
Transcription Factors

Western blot: 1-5 μg/mL. Recognizes the ~43kDa protein.

ELISA: 0.1-1.0 μg/mL

Optimal working dilutions must be determined by end user.

Biochem/physiol Actions

Reacts with CREB.

Physical form

Format: Purified

Purified immunoglobulin. Liquid in PBS containing 0.1% sodium azide.

Preparation Note

Maintain at 2-8°C in undiluted aliquots for up to 6 months after date of receipt.

Analysis Note

Control
Jurkat nuclear extract, A-431 whole cell lysate

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Replaces: 04-218

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 12 - Non Combustible Liquids
• wgk: WGK 2
• flash_point_f: Not applicable
• flash_point_c: Not applicable