1.00591

ELASTIN staining solution acc. to Weigert

for microscopy

• NACRES: NA.41
• UNSPSC Code: 41116124

Properties

• vapor pressure: 59 hPa ( 20 °C)
• Quality Level: 400
• form: liquid
• expl. lim.: 3.5-15 % (v/v) ethanol)
• IVD: for in vitro diagnostic use
• bp: 78.3 °C/1013 hPa (ethanol)
• transition temp: flash point 18 °C
• density: 0.84 g/cm³ at 20 °C
• application(s): clinical testing; diagnostic assay manufacturing; hematology; histology
• storage temp.: 2-8°C

Description

General description

The ELASTIN staining solution acc. to Weigert - for microscopy, is a ready-to-use solution used to detect elastic fibers in connective tissues. Elastic connective-tissue fibers are bundles of glycoprotein-containing microfibrils that accumulate the polymeric protein elastin in their development, gradually resulting in the formation of coarse elastic fibers and membranes. The microfibrils on the surface of the elastic connective-tissue fibers expose high numbers of disulfide groups, which can be chemically detected when sufficient material is available for light-microscopic investigations. Furthermore, they are strongly acidic and thus bind the resorcin-fuchsin dye by electropolarity via interface adsorption.
The resorcin-fuchsin staining method is a regressive method. In tissue sections, nuclei can be stained using a 0.1% nuclear fast red-aluminum sulfate solution (Product number 1.00121) if not only elastic fibers, but also nuclei and the background also need to be visualized.
Brilliant staining results of these fibres are usually achieved when using the ELASTIN staining solution acc. to Weigert - for microscopy in combination with the Picrofuchsin solution acc. van Gieson - for microscopy, Product number 1.00199 and nuclear staining with Weigert′s iron hematoxylin kit - for nuclear staining in histology, Product number 1.15973 respectively Nuclear fast red-aluminium sulfate solution 0.1% - for microscopy, Product number 1.00121.
The 500ml bottle is sufficient for 400 - 500 applications. This product is registered as IVD and CE marked. For more details, please see instructions for use (IFU). The IFU can be downloaded from this webpage.

Analysis Note

Suitability for microscopy: passes test
Nuclei: blue-brown
Collagen: red
Muscle fibers: yellow
Cytoplasm: yellow
Elastic fibers: black

Safety Information

• pictograms: GHS02,GHS05
• signalword: Danger
• hcodes: H225 - H290 - H319
• pcodes: P210 - P233 - P234 - P240 - P241 - P305 + P351 + P338
• Hazard Classifications: Eye Irrit. 2 - Flam. Liq. 2 - Met. Corr. 1
• Storage Class: 3 - Flammable liquids
• wgk: WGK 3
• flash_point_f: 64.4 °F
• flash_point_c: 18 °C