07-146

Anti-Histone H2A (acidic patch) Antibody

serum, Upstate^®

• Synonym(s): H2A histone family, member Q, histone 2, H2ac, histone IIa, histone cluster 2, H2ac
• UNSPSC Code: 12352203
• NACRES: NA.41
• eCl@ss: 32160702
• Conjugate: unconjugated
• Clone: polyclonal
• Application: ChIP, WB
• Citations: 146

Properties

• biological source: rabbit
• Quality Segment: 100
• conjugate: unconjugated
• antibody form: serum
• antibody product type: primary antibodies
• clone: polyclonal
• species reactivity: yeast, chicken, human, rat, Saccharomyces cerevisiae, mouse
• manufacturer/tradename: Upstate^®
• technique(s): ChIP: suitable, western blot: suitable
• isotype: IgG
• NCBI accession no.: NP_003508
• UniProt accession no.: Q16777
• shipped in: dry ice
• target post-translational modification: unmodified
• Gene Information: human ... HIST2H2AC(8338)

Description

General description

14 kDa

Histone H2A is one of the 5 main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N terminal tail H2A is involved with the structure of the nucleosomes of the ′beads on a string′ structure.

Immunogen

KLH-conjugated, synthetic peptide corresponding to amino acids 88-97 (IRNDEELNKL-C) of human Histone H2A.

Application

Anti-Histone H2A (acidic patch) Antibody is a Rabbit Polyclonal Antibody for detection of Histone H2A (acidic patch) also known as histone 2-H2ac, histone IIa & has been validated in ChIP & WB.

Immunofluorescence:
Recommend Anti-phospho-Histone H2A.X (Ser139), clone JBW301, catalog #05-636.

Research Category
Epigenetics & Nuclear Function

Research Sub Category
Histones

Biochem/physiol Actions

Broad species cross-reactivity is expected.

Recognizes Histone H2A, Mr 14 kDa.

Physical form

Rabbit antiserum polyclonal IgG in buffer containing 0.05% sodium azide. Frozen solution.

Unpurified

Preparation Note

Stable for 1 year at -20°C from date of receipt.
For maximum recovery of product, centrifuge the vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing. Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Analysis Note

Control
HeLa cell extract.

Routinely evaluated by western blot on acid extracts from HeLa cell lysate.

Western Blot Analysis:
1:1000-1:5000 dilutions of this lot detected Histone H2A (acidic patch) in acid extracts from HeLa cell lysate.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 12 - Non Combustible Liquids
• wgk: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable