GC Analysis of Volatiles in Roasted Arabica Coffee on SUPELCOWAX 10 after SPME using 50/30 μm DVB/Carboxen/PDMS Fiber

sample/matrix

Arabica coffee beans, roasted, 2 grams with pre-equilibration of 10 min at 60 °C, the entire process under agitation at 500 rpm

SPME fiber

DVB/Carboxen/PD, 50/30 μm, Stableflex (57328-U)

extraction

headspace, 40 min at 60 °C

desorption process

5 min

column

SUPELCOWAX 10, 30 m × 0.25 mm I.D., 0.25 μm (24079)

oven

40 °C (5 min), 4 °C/min to 230 °C, 50 °C/min to 280°C (2 min)

inj. temp.

260 °C

detector

MS (Shimadzu GCMS-QP2010) ionization energy 1.5 kV; mass range: 40-400 amu; scan mode, scan interval : 0.5 sec

MSD interface

230 °C, source temp. 200 °C

carrier gas

helium, 34.0 cm/s and 40.3 KPa

injection

splitless 5 min, then split ratio 1:20

liner

0.75 mm i.d.