- Solubilization of active opiate receptors.
Solubilization of active opiate receptors.
Proceedings of the National Academy of Sciences of the United States of America (1980-08-01)
W F Simonds, G Koski, R A Streaty, L M Hjelmeland, W A Klee
PMID6254034
ANOTACE
Receptors that reversibly bind opiates and opioid peptides have been solubilized from brain and neuroblastoma-glioma hybrid cell NG108-15 membranes. Active receptors are specifically solubilized with a new type of detergent 3-[(3-cholamidopropyl)dimethylammonio]-1-propanesulfonate, which is a zwitterionic derivative of cholic acid. The solubilized receptor complexes behave as large molecules with a Stokes radius of 70 A and contain protein as an essential constituent.