T5525

3,3′,5,5′-Tetramethylbenzidine

TMB substrate, chromogenic, tablet

• Synonyma: BM blue, Sure Blue TMB, TMB, TMB Blotting Plus, TMB substrate
• Lineární vzorec: [-C₆H₂(CH₃)₂-4-NH₂]₂
• Číslo CAS: 54827-17-7
• Molekulová hmotnost: 240.34
• Beilstein/REAXYS Number: 2808541
• MDL number: MFCD00007748
• UNSPSC Code: 12352204
• PubChem Substance ID: 24900293
• NACRES: NA.83

Vlastnosti

• product name: 3,3′,5,5′-Tetramethylbenzidine, tablet, 1 mg substrate per tablet
• form: tablet
• mp: 168-171 °C (lit.)
• storage temp.: 2-8°C
• SMILES string: Cc1cc(cc(C)c1N)-c2cc(C)c(N)c(C)c2
• InChI: 1S/C16H20N2/c1-9-5-13(6-10(2)15(9)17)14-7-11(3)16(18)12(4)8-14/h5-8H,17-18H2,1-4H3
• Inchi Key: UAIUNKRWKOVEES-UHFFFAOYSA-N

Popis

Application

3,3′,5,5′-Tetramethylbenzidine has been used as a substrate in competitive enzyme-linked immunosorbent assay (ELISA) and indirect ELISA.

3,3′,5,5′-Tetramethylbenzidine (TMB) is a noncarcinogenic substitute (Ames test negative) for benzidine suitable as peroxidase substrate for use in ELISA procedures. The substrate produces a soluble end product that is pale blue in color and can be read spectrophotometrically at 370 or 620-650 nm. The TMB reaction may be stopped with 2 M H₂SO₄ (resulting in a yellow color), and read at 450 nm. A sensitive and specific reagent for the detection of blood, assay of hemoglobin, assay of peroxidases.

Bezpečnostní informace

• pictograms: GHS08,GHS07
• signalword: Warning
• hcodes: H302,H351,H413
• pcodes: P202 - P264 - P273 - P280 - P301 + P312 - P308 + P313
• Hazard Classifications: Acute Tox. 4 Oral - Aquatic Chronic 4 - Carc. 2
• Storage Class: 11 - Combustible Solids
• wgk_germany: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: dust mask type N95 (US), Eyeshields, Faceshields, Gloves