T2928
Monoclonal Anti-Tyrosine Hydroxylase antibody produced in mouse
clone TH-16, ascites fluid
Synonyma:
Anti-DYT14, Anti-DYT5b, Anti-TYH
Přihlásitk zobrazení cen stanovených pro organizaci a smluvních cen
About This Item
Doporučené produkty
biological source
mouse
Quality Level
conjugate
unconjugated
antibody form
ascites fluid
antibody product type
primary antibodies
clone
TH-16, monoclonal
contains
15 mM sodium azide
species reactivity
rat, sheep, human, monkey, bovine, rabbit, guinea pig
technique(s)
immunohistochemistry: suitable
immunoprecipitation (IP): suitable using native or SDS-denatured TH.
microarray: suitable
western blot: 1:8,000 using extracts of cultured rat adrenal pheochromocytoma (PC12) cells.
isotype
IgG1
UniProt accession no.
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
General description
Monoclonal Anti-Tyrosine Hydroxylase (mouse IgG1 isotype) is derived from the TH-16 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from immunized BALB/c mice. Tyrosine hydroxylase (TH) exists in four isoforms in humans. They are are TH-1 (M.W. 55,600), TH-2 (4 additional amino acids; M.W. 56,000), TH-3, (27 additional amino acids; M.W. 58,100), and TH-4 (4 plus 27 additional amino acids; 58,500). Only humans express all four isoforms; all other anthropoids express only the TH-1 and TH-2 isoforms.
Tyrosine hydroxylase (TH) is encoded by the gene mapped to human chromosome 11p15. TH is a tetramer of four identical subunits, which is characterized with a regulatory, catalytic and tetramerization domains. The enzyme utilizes tyrosine, tetrahydrobiopterin (BH4) and O2 as cosubstrates, and Fe2+ as a cofactor.
Specificity
The antibody recognizes an epitope present in the N-terminal region (between aa 40-152) of both rodent and human tryosine hydroxylase. TH-16 detects both the intact subunits and the 59/57 kDa doublet and an array of decreasing molecular weight TH forms resulting from severe proteolysis.
Immunogen
rat tyrosine hydroxylase (TH).
Application
Monoclonal Anti-Tyrosine Hydroxylase antibody produced in mouse has also been used in
- western blotting
- immunofluorescence labelling
- immunohistochemical staining
- enzyme linked immunosorbent assay (ELISA)
- dot blot
- immunoprecipitation
- immunocytochemistry
Monoclonal Anti-tyrosine hydroxylase antibody produced in mouse has been used in immunohistochemistry and double immunofluorescence labeling.
Biochem/physiol Actions
Tyrosine hydroxylase (TH) catalyzes the first rate limiting step in the biosynthesis of catecholamine neurotransmitter i.e. the conversion of L-tyrosine to L-dopa. Inhibition of TH by L-phenylalanine, might play a crucial role in phenylketonuria and block the synthesis of norepinephrine. Activity of TH can be regulated by phosphorylation. Decreased expression of TH is associated with various neuropsychiatric diseases such as schizophrenia and Parkinson′s disease (PD).
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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recommended
Storage Class
10 - Combustible liquids
wgk_germany
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
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