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SAE0013

Oxyrase ® for Broth

Synonym(s):

OB

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Size/SKUAvailabilityPrice
50 mL
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$89.90
100 mL
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$139.00
6 x 10 mL
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$154.00

About This Item

NACRES:
NA.54
UNSPSC Code:
12352204

$89.90


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Quality Segment

shipped in

dry ice

storage temp.

−20°C

General description

Oxyrase for Broth is a sterile enzyme formulation that uses the Oxyrase Enzyme System to produce anaerobic conditions in different bacteriological broth media. Oxyrase for Broth facilitates growth of anaerobes, such as for reviving lyophiles. Oxyrase for Broth is a ready-to-use product that renders broth anaerobic in 10-15 minutes, and continues to remove oxygen from the container headspace beyond that time.

Application

Oxyrase® for broth has been used in reinforced clostridial media to provide an anaerobic environment for culturing Bifidobacterium adolescentis.[1] It has also been used as a component in PMM/imaging media for oxygen-free microscopy experiments to remove oxygen.[2]

Biochem/physiol Actions

Oxyrase® for broth is useful to cultivate species like Shigella sonnei and Shewanella oneidensis.[3][4]It also provides anaerobic conditions for cultivating species like Escherichia coli, Staphylococcus aureus, and Pseudomonas aeruginosa.[5]

Preparation Note

Store at -20 °C. (Long Term Storage)

Analysis Note

Oxyrase contains a penicillin binding protein that may interfere with penicillin and some related antibiotics.

Legal Information

Oxyrase is a registered trademark of Oxyrase, Inc.

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This Item
SAE0058Y137570122
shipped in

dry ice

shipped in

-

shipped in

-

shipped in

-

Quality Level

200

Quality Level

100, 200

Quality Level

200

Quality Level

200

storage temp.

−20°C

storage temp.

−20°C

storage temp.

-

storage temp.

2-25°C


Storage Class

10 - Combustible liquids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable



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Questions

1–2 of 2 Questions  
  1. Precautions for use and storage of oxyrase?

    1 answer
    1. The storage and handling of this material can be found on the material Safety Datasheet. This product should be stored at -20 °C. For safety information, please refer to the SDS by using the link below:
      https://www.sigmaaldrich.com/documents-search?tab=sds

      Helpful?

  2. What is the recommended concentration of oxyrase in broth? I plan to add it to Todd Hewitt broth for better growing strep species. Once the oxyrase in TH broth is made, at what temperature should it be stored? And how long will it be viable? Thanks!

    1 answer
    1. The recommended concentration of oxyrase in broth is 0.1 mL of the product added aseptically to each 1.0 mL of prepared sterile broth medium. After preparation, the oxyrase in Todd Hewitt broth should be stored at 4 °C. The viability of the product can vary, but it is generally recommended to use it within a few weeks for optimal performance.

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