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Key Documents

S6782

Sigma-Aldrich

Superdex®

Superdex®200 Prep Grade

Synonyma:

Size exclusion media, Superdex column

Přihlásitk zobrazení cen stanovených pro organizaci a smluvních cen
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About This Item

MDL number:
MFCD00166816
UNSPSC Code:
23151817
NACRES:
NA.56

form

suspension (20% aqueous ethanol)

technique(s)

protein array: suitable

matrix

cross-linked agarose

particle size

24-44 μm

pore size

1-100 kDa MW range (dextrans)
10-600 kDa MW range (proteins)

operating pH range

1-14(short term)
3-12(long term)

storage temp.

2-8°C

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General description

Combines the high selectivity of a Sephadex matrix with the chemical and physical stability of crosslinked agarose.

Application

Superdex® is used for protein chromatography, gel filtration chromatography, gel filtration media, resins and separation media. Superdex® has been used for the purification and characterization of a novel laccase from the edible mushroom Hericium coralloides. Superdex® has also been used for the purification and mechanism exploration of a potential human hepatocellular carcinoma inhibitor from Bauhinia purpurea L. seeds.
Optimized for high resolution preparative separations by size exclusion chromatography. Recommended fractionation ranges are listed for globular proteins and dextrans.

Legal Information

Superdex is a registered trademark of Cytiva

pictograms

Flame
GHS02

signalword

Warning

hcodes

H226

Hazard Classifications

Flam. Liq. 3

Storage Class

3 - Flammable liquids

wgk_germany

WGK 3

flash_point_f

104.0 °F

flash_point_c

40 °C

Osvědčení o analýze (COA)

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J Oh et al.
Human molecular genetics, 9(3), 375-385 (2000-02-03)
Hermansky-Pudlak syndrome (HPS) is a rare autosomal recessive disorder in which oculocutaneous albinism, bleeding tendency and a ceroid-lipofuscin lysosomal storage disease result from defects of multiple cytoplasmic organelles: melanosomes, platelet dense granules and lysosomes. The HPS polypeptide, a 700 amino
J L Van Hove et al.
Biochemistry and molecular biology international, 43(3), 613-623 (1997-11-14)
Large quantities of recombinant acid alpha-glucosidase are needed for in vivo experimentation of enzyme replacement therapy in Pompe disease. We describe a new purification method for the purification of this recombinant enzyme from tissue culture medium consisting of concanavalin A
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Infection and immunity, 76(7), 3164-3169 (2008-04-23)
Despite effective chemotherapy, schistosomiasis remains a major public health problem in the developing world, with at least 200 million active infections resulting in significant morbidity. Rapid reinfection after treatment, accompanied by extensive residual morbidity, mandates alternative control strategies, including vaccine
Thomas Ouellette et al.
Protein expression and purification, 30(2), 156-166 (2003-07-26)
A recombinant form of human rhIL-7 was overexpressed in Escherichia coli HMS174 (DE3) pLysS under the control of a T7 promoter. The resulting insoluble inclusion bodies were separated from cellular debris by cross-flow filtration and solubilized by homogenization with 6
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Elling, L., and Kula, M.-R.
Journal of Biotechnology, 29(3), 277-286 (1993)
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