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RAB0713

Human MSLN / Mesothelin ELISA Kit

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About This Item

NACRES:
NA.32
UNSPSC Code:
41116158
Gene information:
human ... MSLN(10232)
Input:
sample type plasma
sample type cell culture supernatant(s)
sample type serum
Species reactivity:
human
Storage temp.:
−20°C
Shipped in:
wet ice
Pricing and availability is not currently available.
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species reactivity

human

packaging

kit of 96 wells (12 strips x 8 wells)

technique(s)

ELISA: suitable

input

sample type plasma
sample type cell culture supernatant(s)
sample type serum

assay range

inter-assay cv: <12%
intra-assay cv: <10%
sensitivity: 100 pg/mL
standard curve range: 0.123-30 ng/mL

detection method

colorimetric

shipped in

wet ice

storage temp.

−20°C

Gene Information

human ... MSLN(10232)

General description

The MSLN ELISA kit provides for the Quantitative measurement of Mesothelin in Cell Culture Supernatants, Plasma and Serum.

Application

For research use only. Not for use in diagnostic procedures.
Please refer to the attached General ELISA KIT Procedure (sandwich, competitive & Indirect ELISA)

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species reactivity

human

species reactivity

human

species reactivity

human

species reactivity

human

Gene Information

human ... MSLN(10232)

Gene Information

human ... MB(4151)

Gene Information

human ... SHH(6469)

Gene Information

human ... FSTL3(10272)

assay range

inter-assay cv: <12%
intra-assay cv: <10%
sensitivity: 100 pg/mL
standard curve range: 0.123-30 ng/mL

assay range

inter-assay cv: <12%
intra-assay cv: <10%
sensitivity: 0.82 ng/mL
standard curve range: 0.819-200 ng/mL

assay range

inter-assay cv: <12%
intra-assay cv: <10%
sensitivity: 42 pg/mL
standard curve range: 40.96-10000 pg/mL
(Assay Diluent C), inter-assay cv: <12%
intra-assay cv: <10%
sensitivity: 8 pg/mL
standard curve range: 40.96-10000 pg/mL
(Assay Diluent B)

assay range

inter-assay cv: <12%
intra-assay cv: <10%
sensitivity: 250 pg/mL
standard curve range: 204.8-50000 pg/mL

input

sample type plasma
sample type cell culture supernatant(s)
sample type serum

input

sample type plasma
sample type serum
sample type cell culture supernatant(s)

input

sample type cell culture supernatant(s)
sample type serum
sample type urine
sample type plasma

input

sample type plasma
sample type urine
sample type cell culture supernatant(s)
sample type serum

technique(s)

ELISA: suitable

technique(s)

ELISA: suitable

technique(s)

ELISA: suitable, capture ELISA: suitable

technique(s)

ELISA: suitable, capture ELISA: suitable

detection method

colorimetric

detection method

colorimetric

detection method

colorimetric

detection method

colorimetric

Kit Components Also Available Separately

Product No.
Description
SDS & Pricing
  • RABTMB3
    ELISA Colorimetric TMB Reagent (HRP Substrate, Item H)
    SDS
  • RABSTOP3
    ELISA Stop Solution (Item I)
    SDS
  • RABWASH4
    20X Wash Buffer (Item B)
    SDS

pictograms

Corrosion
GHS05

signalword

Warning

hcodes

H290

Hazard Classifications

Met. Corr. 1

Storage Class

10 - Combustible liquids

flash_point_f

Not applicable

flash_point_c

Not applicable

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Questions

1–2 of 2 Questions  

  1. Which amino acid residues of the MSLN protein does the antibody bind to? Does the ELISA kit recognize the mature form of MLSN protein only (~40kDa) or does it also bind to MPF?

    1 answer

    1. The epitope mapping has not been performed for the antibodies used in this kit. The antibody and standard information is included below:


      Capture Antibody Source:
      Monoclonal Mouse IgG1 Immunogen: Mouse myeloma cell line NS0-derived recombinant human Mesothelin
      Accession# AAH09272

      Detection Antibody Source:
      Polyclonal Goat IgG Immunogen: Mouse myeloma cell line NS0-derived recombinant human Mesothelin isoform 1 (aa296-580)
      Accession# ABW03459

      Standard Recombinant Protein Source:
      Mouse myeloma cell line, NS0-derived human Mesothelin protein aa296-580 Accession# AAH09272

      No additional information is available regarding the variant detected by the antibodies.

      Helpful?

  2. Could you provide the molecular weight of the standard protein used in the Human MSLN ELISA (RAB0713-1KT) kit?

    1 answer

    1. The predicted molecular weight for the Mesothelin-1 standard is 33 kDa.

      Helpful?

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