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Key Documents

R8508

Sigma-Aldrich

Ribonucleic acid, transfer from baker′s yeast (S. cerevisiae)

buffered aqueous solution

Synonyma:

Transfer RNA, tRNA

Přihlásitk zobrazení cen stanovených pro organizaci a smluvních cen


About This Item

Číslo CAS:
MDL number:
UNSPSC Code:
12352106
NACRES:
NA.55

grade

for molecular biology

Quality Level

form

buffered aqueous solution

concentration

9-11 mg/mL

foreign activity

DNase, Nickase, none detected

shipped in

dry ice

storage temp.

−20°C

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General description

Transfer RNA (tRNA) is isolated from baker′s yeast.
Transfer RNA (tRNA) is a short nucleotide RNA chain, which has an L-shaped structure.

Application

Ribonucleic acid, transfer from baker′s yeast (S. cerevisiae) has been used to precipitate mRNA of denuded oocytes and for reverse transcription. It has also been used to seal magnetic beads in RNA pull-down assay of nasopharyngeal carcinoma (NPC) cells.
Ribonucleic acid, transfer from baker′s yeast (S. cerevisiae) is suitable for use as a carrier in nucleic acid purification and precipitation.

Biochem/physiol Actions

Transfer RNA (tRNA) acts as an ‘adaptor′ molecule that translates the three-nucleotide codon sequence in the mRNA to the corresponding amino acid of that codon. It determines the genetic code by linking amino acids and nucleic acids. tRNA is involved in viral replication, bacterial cell wall biosynthesis, cell stress response, and animal behavior control. Aberrations in tRNA molecules are associated with several human diseases.

Components

tRNA is provided in a solution in 10 mM Tris HCl (pH 7.4) in 1 mM EDTA.

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)


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