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MBD0015

DAPI ready made solution

For Nuclear counterstain in immunoflourecence microscopy, High Content Screening (HCS), Chromosome staining and flow cytometry (FACS)., 1 mg/mL

DAPI ready made solution

Synonym(s):

4′,6-Diamidino-2-phenylindole dihydrochloride, DAPI Dye, DAPI Stain, DNA Content Counterstain, 2-(4-Amidinophenyl)-6-indolecarbamidine dihydrochloride, DAPI dihydrochloride

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Size/SKUAvailabilityPrice
1 mL

Available to ship TODAYfromMILWAUKEE

$89.20
5 mL

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$420.00
10 mL

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$758.00
50 mL

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$2,350.00
$1,997.50

About This Item

Empirical Formula (Hill Notation):
C16H15N5 · 2HCl
CAS Number:
28718-90-3
Molecular Weight:
350.25
NACRES:
NA.55
UNSPSC Code:
41105500
Beilstein/REAXYS Number:
4894417
MDL number:
MFCD00012681

$89.20

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Quality Segment

200

form

liquid

storage condition

protect from light

concentration

1 mg/mL

technique(s)

transfection: suitable

solubility

water: soluble

fluorescence

λex 340 nm; λem 488 nm (nur DAPI), λex 364 nm; λem 454 nm (DAPI-DNA-Komplex)

suitability

suitable for nucleic acid staining

shipped in

dry ice

storage temp.

−20°C

SMILES string

Cl.Cl.NC(=N)c1ccc(cc1)-c2cc3ccc(cc3[nH]2)C(N)=N

InChI

1S/C16H15N5.2ClH/c17-15(18)10-3-1-9(2-4-10)13-7-11-5-6-12(16(19)20)8-14(11)21-13;;/h1-8,21H,(H3,17,18)(H3,19,20);2*1H

InChI key

FPNZBYLXNYPRLR-UHFFFAOYSA-N

General description

DAPI, 4′,6-diamidino-2-phenylindole, is a blue fluorescent nucleic acid stain that fluoresces brightly upon selectively binding to the minor groove of double stranded DNA. Its selectivity for DNA and high cell permeability allows efficient staining of nuclei with little background from the cytoplasm. The excitation and emission wavelengths of DAPI-DNA complex are 360 nm and 460 nm, respectively.

Application

DAPI Readymade Solution is provided at 1mg/mL concentration. It is recommended to dilute DAPI readymade solution 1:1000 with a desired buffer for a 1μg/mL working concentration (typical working concentration used in most applications is 0.1μg/mL-10μg/mL). Store DAPI solution (1mg/mL) -20°C protected from light. It is recommended to aliquot DAPI readymade solution and to avoid freeze thaw cycles.

DAPI is several times more sensitive than ethidium bromide for staining DNA in agarose gels. It may be used for photofootprinting of DNA, to detect annealed probes in blotting applications by specifically visualizing the double-stranded complex, and to study the changes in DNA and analyze DNA content during apoptosis using flow cytometry. DAPI staining has also been shown to be a sensitive and specific detection method for mycoplasma.

For Nuclear counterstain in immunoflourecence microscopy, High Content Screening (HCS), Chromosome staining and flow cytometry (FACS).
DAPI is several times more sensitive than ethidium bromide for staining DNA in agarose gels.
DAPI is several times more sensitive than ethidium bromide for staining DNA in agarose gels. It may be used for photofootprinting of DNA, to detect annealed probes in blotting applications by specifically visualizing the double-stranded complex, and to study the changes in DNA and analyze DNA content during apoptosis using flow cytometry. DAPI staining has also been shown to be a sensitive and specific detection method for mycoplasma.

Biochem/physiol Actions

Cell permeable fluorescent minor groove-binding probe for DNA. Binds to the minor groove of double-stranded DNA (preferentially to AT rich DNA), forming a stable complex which fluoresces approximately 20 times greater than DAPI alone.

Features and Benefits

  • Ready-to-use liquid, cell permeable dye.
  • Can be used with live cells and fixed cells.
  • Effective for fixed-cell staining and quantitation of DNA content.
  • Optimal Counterstain when using fluorescent antibodies during fluorescence microscopy or high-content screening (HCS) applications.

Physical form

DAPI readymade solution is provided at 1mg/mL concentration in water

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This Item
D9542102362760015.08741
DAPI ready made solution For Nuclear counterstain in immunoflourecence microscopy, High Content Screening (HCS), Chromosome staining and flow cytometry (FACS)., 1 mg/mL

Sigma-Aldrich

MBD0015

DAPI ready made solution

DAPI for nucleic acid staining

Sigma-Aldrich

D9542

DAPI

DAPI 4′,6-Diamidine-2′-phenylindole dihydrochloride

Roche

10236276001

DAPI

DAPI Stain

Millipore

5.08741

DAPI Stain

suitability

suitable for nucleic acid staining

suitability

suitable for fluorescence

suitability

-

suitability

-

technique(s)

transfection: suitable

technique(s)

transfection: suitable

technique(s)

-

technique(s)

-

fluorescence

λex 340 nm; λem 488 nm (nur DAPI), λex 364 nm; λem 454 nm (DAPI-DNA-Komplex)

fluorescence

λex 340 nm; λem 488 nm (nur DAPI), λex 364 nm; λem 454 nm (DAPI-DNA-Komplex)

fluorescence

λex 340 nm; λem 488 nm (nur DAPI), λex 364 nm; λem 454 nm (DAPI-DNA-Komplex)

fluorescence

λex ~359 nm, λem ~461 nm

concentration

1 mg/mL

concentration

-

concentration

-

concentration

-

form

liquid

form

powder

form

powder

form

liquid

solubility

water: soluble

solubility

H2O: 20 mg/mL, PBS: insoluble

solubility

-

solubility

-

pictograms

Exclamation mark
GHS07

signalword

Warning

hcodes

H317

Hazard Classifications

Skin Sens. 1

Storage Class

12 - Non Combustible Liquids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

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Questions

1–2 of 2 Questions  

  1. What concentration should be use for live/dead cell staining for live cells in FACS sorter, to be able to culture back the sorted cells?

    1 answer

    1. The optimum concentration varies, depending on the cell type. However, a concentration of 0.1-1.0 µg/mL is generally sufficient for live/dead FACS sorting. Titrate DAPI to determine the concentration best suited for the cell type.

      The following paper with protocols that may be useful for live/dead FACS sorting:
      https://onlinelibrary.wiley.com/doi/10.1002/eji.201646632

      Helpful?

  2. Is this product sterile?

    1 answer

    1. Product MBD0015 is not a sterile. This solution is at a 1 mg/mL concentration. Required volumes may be diluted with PBS or water and filter sterilized if desired.

      Helpful?

Reviews

Active Filters

  1. Egypt
    • Review 1
    • Votes 0
    1 out of 5 stars.

    The product is not working on frozen sections

    I bought this product to stain frozen sections however the product did not stain frozen sections. The R and D team did not have specific recommendation.

    Helpful?

    1. Response from MilliporeSigma:

      Thank you for your review. We are sorry to hear that your experience did not match your product expectations. We encourage customers who experience problems with our products to call or email us for additional technical support. Please visit https://www.sigmaaldrich.com/US/en/support/customer-support to submit a Product Technical Inquiry.