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LUC1

Sigma-Aldrich

Luciferase Reporter Gene Detection Kit

Přihlásitk zobrazení cen stanovených pro organizaci a smluvních cen
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About This Item

UNSPSC Code:
12352207
NACRES:
NA.84

usage

 kit sufficient for 100 assays

storage temp.

−70°C

General description

Firefly luciferase is one of the most utilized reporter genes for the study of gene expression. It is an extremely sensitive, rapid, and easy-to-use reporter gene. The chemiluminescent reaction catalyzed by luciferase is one of the most sensitive analytical tools for measuring gene expression. The luciferase substrate contains coenzyme A for increased and sustained luminescence compared to conventional methods. This eliminates the need for automated luminometer injection of the substrate and allows analysis by photographic film or scintillation counting. The lysis buffer contains polymyxin B which further enhances the signal and eliminates lysozyme treatment and freeze-thawing of cells. Cell lysis buffer is compatible with β-galactosidase assays. The enzyme encoded by the luciferase reporter gene catalyzes the oxidation of D-luciferin in the presence of ATP, oxygen, and Mg2+. The fluorescent product is quantified to measure its activity.

pictograms

CorrosionEnvironment
GHS05,GHS09

signalword

Danger

hcodes

H318,H410

Hazard Classifications

Aquatic Acute 1 - Aquatic Chronic 2 - Eye Dam. 1

Storage Class

10 - Combustible liquids

flash_point_f

Not applicable

flash_point_c

Not applicable

Osvědčení o analýze (COA)

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Gerben van Ooijen et al.
Current biology : CB, 21(10), 869-875 (2011-05-03)
Circadian clocks were, until recently, seen as a consequence of rhythmic transcription of clock components, directed by transcriptional/translational feedback loops (TTFLs). Oscillations of protein modification were then discovered in cyanobacteria. Canonical posttranslational signaling processes have known importance for clocks across
Rachel S Edgar et al.
Nature, 485(7399), 459-464 (2012-05-25)
Cellular life emerged ∼3.7 billion years ago. With scant exception, terrestrial organisms have evolved under predictable daily cycles owing to the Earth's rotation. The advantage conferred on organisms that anticipate such environmental cycles has driven the evolution of endogenous circadian rhythms
Susan L Kozak et al.
The Journal of biological chemistry, 281(39), 29105-29119 (2006-08-05)
Deoxycytidine deaminases APOBEC3G (A3G) and APOBEC3F (A3F) (members of the apolipoprotein B mRNA-editing catalytic polypeptide 3 family) have RNA-binding motifs, invade assembling human immunodeficiency virus (HIV-1), and hypermutate reverse transcripts. Antagonistically, HIV-1 viral infectivity factor degrades these enzymes. A3G is
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Scientific reports, 10(1), 18427-18427 (2020-10-30)
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Sortimentní položky

Bioluminescent Firefly Luciferase Assays

Firefly luciferase is a sensitive reporter for gene studies due to its absence in mammalian cells or tissues.

Bioluminiscenční testy světlušky luciferázy

Světluška luciferáza je citlivým reportérem pro genové studie, protože se v savčích buňkách nebo tkáních nevyskytuje.

Náš tým vědeckých pracovníků má zkušenosti ve všech oblastech výzkumu, včetně přírodních věd, materiálových věd, chemické syntézy, chromatografie, analytiky a mnoha dalších..

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