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H9408

Sigma-Aldrich

β-Hydroxybutyrate Dehydrogenase from Pseudomonas lemoignei

lyophilized powder, ≥200 units/mg protein

Synonyma:

(R)-3-Hydroxybutanoate:NAD+ oxidoreductase, 3-HBDH

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About This Item

Číslo CAS:
9028-38-0
Číslo enzymu podle klasifikace EK:
1.1.1.30 (BRENDA, IUBMB)
MDL number:
MFCD00130633
UNSPSC Code:
12352204
NACRES:
NA.54

biological source

bacterial (Pseudomonas lemoignei)

Quality Level

200

form

lyophilized powder

specific activity

≥200 units/mg protein

purified by

chromatography

foreign activity

lactic dehydrogenase ≤0.05%
malic dehydrogenase ≤0.1%

storage temp.

−20°C

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General description

D-β-hydroxybutyrate dehydrogenase (BDH) is a membrane bound lipid-requiring enzyme.

Application

β-Hydroxybutyrate Dehydrogenase from Pseudomonas lemoignei has been used in coupled assay to confirm the formation of acetoacetate.
Suitable for the determination of acetoacetate and D(-)-3-hydroxybutyrate by the method of Williamson, D. H., and Mellanby, J., Methods of Enzymatic Analysis, Bergmeyer, H., ed., 2nd edition, 4, 1836 (1974).

Biochem/physiol Actions

In Paracoccus denitrificans, 3-hydroxybutyrate dehydrogenase plays a key role in the degradation of intracellular polyhydroxybutyrate and polyhydroxyvalerate.
In mammalian systems, β-hydroxybutyrate dehydrogenase is localized on the inner mitochondrial membrane and requires phosphatidyl choline for activity. In contrast, the enzyme from Pseudomonas is a soluble cytosolic enzyme that does not require a phospholipid allosteric activator. The enzyme is required for the utilization of ketone bodies as a source of metabolic energy. It catalyzes the oxidation of 3-hydroxybutyrate to acetoacetate, the first step in the conversion of ketone bodies to citric acid, which is then further metabolized via the tricarboxylic acid cycle (Krebs cycle).

Unit Definition

One unit will oxidize 1.0 μmole of D-β-hydroxybutyrate to acetoacetate per min at pH 7.8 at 37 °C.

Physical form

lyophilized powder containing sucrose, β-NAD and Tris buffer salts

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

Osvědčení o analýze (COA)

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D-beta-HYDROXYBUTYRATE DEHYDROGENASE: A MOLECULAR BIOLOGY APPROACH TO THE STUDY OF A LIPID-REQUIRING ENZYME
Structure and Function of Membrane Proteins, 283-300 (1983)
3-Hydroxybutyrate oligomer hydrolase and 3-hydroxybutyrate dehydrogenase participate in intracellular polyhydroxybutyrate and polyhydroxyvalerate degradation in Paracoccus denitrificans
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