H3537

HEPES solution

99.5%, liquid, BioPerformance Certified, 1 M, suitable for cell culture, 0.2 μm filtered

• Synonyma: N-(2-Hydroxyethyl)piperazine-N′-(2-ethanesulfonic acid)
• Číslo CAS: 7365-45-9
• UNSPSC Code: 12161700
• PubChem Substance ID: 329815199
• NACRES: NA.25
• MDL number: MFCD00006158

Vlastnosti

• Název produktu: HEPES solution, BioPerformance Certified, 1 M, suitable for cell culture, 0.2 μm filtered
• grade: BioPerformance Certified
• Quality Segment: 200
• sterility: 0.2 μm filtered
• form: liquid
• concentration: 1 M
• technique(s): cell culture | mammalian: suitable
• impurities: Bioburden, tested, DNase, RNase, Protease, Nickase, free, endotoxin, tested
• pH: 5.0-6.0
• useful pH range: 6.8-8.2
• cation traces: Fe: <5 ppm, heavy metals (as Pb): <5 ppm
• suitability: suitable for molecular biology
• application(s): diagnostic assay manufacturing
• SMILES string: OCCN1CCN(CCS(=O)(O)=O)CC1
• InChI: 1S/C8H18N2O4S/c11-7-5-9-1-3-10(4-2-9)6-8-15(12,13)14/h11H,1-8H2,(H,12,13,14)
• InChI key: JKMHFZQWWAIEOD-UHFFFAOYSA-N

Popis

General description

HEPES has been described as one of the best all-purpose buffers available for biological research. At biological pH, the molecule is zwitterionic, and is effective as a buffer at pH 6.8 to 8.2. HEPES has been used in a wide variety of applications, including tissue culture. It is commonly used to buffer cell culture media in air. HEPES finds its usage in in vitro experiments on Mg.

Application

RNAse HEPES has been used:

• To supplement Dulbecco′smodified Eagle′smedium for maintenance of cell line and RPMI medium to wash rat islets
• To recover purified ribonucleotide
• As a component of HEPES/KOH buffer and adenylation buffer for small RNA isolation and sequencing
• As a component in buffers used for nuclear extract preparation and also to supplement RPMI-1640 medium for maintenance of islets

Bezpečnostní informace

• Skladovací třída: 10 - Combustible liquids
• wgk: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable