For an MTT assay, standard RPMI or DMEM media are suitable options. These include RPMI (Product R0883) and DMEM (Product D5671), both of which may contain up to 10% FBS. When performing assays, such as mitogen or complement-mediated cytotoxicity assays, cells should be suspended in a tissue culture medium, such as DMEM or RPMI, before adding the MTT reagent to each well.
D5030
Dulbecco′s Modified Eagle′s Medium
Without glucose, L-glutamine, phenol red, sodium pyruvate and sodium bicarbonate, powder, suitable for cell culture
Synonyma:
DME, DMEM
Přihlásit pro zobrazení organizačních a smluvních cen.
Vybrat velikost
Změnit zobrazení
| Velikost balení | Skladová položka | Dostupnost | Cena |
|---|---|---|---|
| 10 x 1 L | Obraťte se na zákaznický servis a vyžádejte si informaci o dostupnosti. | 4 250,00 Kč | |
| 10 L | Obraťte se na zákaznický servis a vyžádejte si informaci o dostupnosti. | 4 250,00 Kč | |
| 50 L | Obraťte se na zákaznický servis a vyžádejte si informaci o dostupnosti. | 17 400,00 Kč |
O této položce
NACRES:
NA.75
UNSPSC Code:
12352207
4 250,00 Kč
Obraťte se na zákaznický servis a vyžádejte si informaci o dostupnosti.
Technický servis
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Dovolte nám, abychom vám pomohliQuality Level
form
powder
technique(s)
cell culture | mammalian: suitable
components
phenol red: no
NaHCO3: no
L-glutamine: no
HEPES: no
glucose: no
sodium pyruvate: no
shipped in
ambient
storage temp.
2-8°C
General description
Dulbecco′s Modified Eagle′s Medium (DME) is a modification of Basal Medium Eagle (BME) that has been formulated with a 4-fold higher concentration of amino acids and vitamins. It includes additional supplementary components. The DME formula, first reported for culturing embryonic mouse cells, contained 1,000 mg/L of glucose. Modifying the medium with 4,500 mg/L glucose is optimal for culturing certain cell types.
The most basic formulation offered. This formulation is used by investigators who want to start with the essential components of DME, and have the flexibility to optimize the formula for their own application.
Application
Dulbecco′s Modified Eagle′s Medium has been used:
- to culture the isolated flexor digitorum brevis (FDB) fibers to measure exogenous fatty acid (FA) utilization as part of oxygen consumption rate (OCR) measurements
- to culture the isolated tibialis anterior (TA) muscle fibers for lactate measurements
- to culture the human bone marrow mesenchymal stem cells for osteogenic differentiation to prepare a low-glucose medium to culture human hepatocellular carcinoma HepG2 cells
Preparation Note
Formulated to contain 8.3 grams of powder per liter of medium.
Supplement with 1.0 g/L glucose, 0.584 g/L L-glutamine, 3.7 g/L sodium bicarbonate.
1 of 1
Tato položka | |||
|---|---|---|---|
| form powder | form liquid | form powder | form powder |
| technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable |
| components phenol red: no | components phenol red: no | components L-glutamine: yes | components phenol red: yes |
| shipped in ambient | shipped in ambient | shipped in ambient | shipped in ambient |
| storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C |
| Quality Level 500 | Quality Level 400 | Quality Level 500 | Quality Level 500 |
signalword
Warning
hcodes
Hazard Classifications
Eye Irrit. 2
Skladovací třída
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
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Protokoly
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Sortimentní položky
Calcium is an ionically stable divalent cation with important beneficial and toxic properties in cell culture. It is a component of a wide range of cell culture media.
Související obsah
Our broad range of the most trusted tools for cell culture includes stringently sourced and tested FBS, established media formulations, and sterile labware. Cutting-edge techniques using stem cells and 3D matrices are enabled by organoids, hydrogels, culture scaffolds, and bioinks for 3D bioprinting.
Instructions
Eunyong Ahn et al.
Molecular systems biology, 13(11), 953-953 (2017-11-08)
Cellular metabolic demands change throughout the cell cycle. Nevertheless, a characterization of how metabolic fluxes adapt to the changing demands throughout the cell cycle is lacking. Here, we developed a temporal-fluxomics approach to derive a comprehensive and quantitative view of
Nataly Stylianou et al.
Oncogene, 38(7), 913-934 (2018-09-09)
The propensity of cancer cells to transition between epithelial and mesenchymal phenotypic states via the epithelial-mesenchymal transition (EMT) program can regulate metastatic processes, cancer progression, and treatment resistance. Transcriptional investigations using reversible models of EMT, revealed the mesenchymal-to-epithelial reverting transition
Zhongjie Fu et al.
EMBO molecular medicine, 10(1), 76-90 (2017-11-29)
The neural cells and factors determining normal vascular growth are not well defined even though vision-threatening neovessel growth, a major cause of blindness in retinopathy of prematurity (ROP) (and diabetic retinopathy), is driven by delayed normal vascular growth. We here
Globální číslo obchodní položky
| Skladová položka | GTIN |
|---|---|
| D5030-50K | 04061834180272 |
| D5030-10X1L | 04061833585979 |
| D5030-10L | 04061833585962 |
| D5030-50L | 04061833585986 |
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Is there an available variant of EMEM that is free from phenol red, glutamine, and NaHCO3? Additionally, for an MTT assay, would it be necessary to have two types of media – one being the aforementioned EMEM variant and the other for regular culture growth?
1 answer-
Helpful?
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Is the CaCl2 included in Product D5030 in the form of a dihydrate or is it anhydrous?
1 answer-
The formulation utilizes Calcium Chloride Anhydrous rather than the dihydrate form.
Helpful?
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What are the preparation instructions of the 10L product?
1 answer-
As listed on the product detail page in the 'DESCRIPTION' section under 'Reconstitution', this product can be supplemented with 1.0 g/L glucose, 0.584 g/L L-glutamine, 3.7 g/L sodium bicarbonate.
Helpful?
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How can we reconstitute this powder to create ready-to-use media? What is the reason for excluding Sodium bicarbonate from powdered media?
How can we reconstitute this powder to create ready-to-use media? What is the reason for excluding Sodium bicarbonate from powdered media?
0 answers