72464

Atto 565 NHS ester

BioReagent, suitable for fluorescence, ≥90% (HPLC)

• Empirical Formula (Hill Notation): C₃₅H₃₄ClN₃O₁₁
• Molecular Weight: 708.11
• UNSPSC Code: 12352108
• NACRES: NA.32
• MDL number: MFCD03456131

Properties

• product line: BioReagent
• Quality Segment: 100
• assay: ≥90% (HPLC), ≥90% (degree of coupling)
• form: powder
• manufacturer/tradename: ATTO-TEC GmbH
• suitability: suitable for fluorescence
• detection method: fluorometric
• storage temp.: −20°C

Description

General description

Atto 565 NHS ester is a commonly used amine-reactive reagent, provided by ATTO-TEC. It is widely used in protein labeling and readily reacts with compounds containing amino groups, forming a chemically stable amide bond between the dye, e.g., a protein. The optimum pH-range for NHS-ester coupling is pH 8.0 – 9.0. At this pH amino groups of proteins, i.e., the ε-amino groups of lysines are unprotonated to a degree sufficiently high for fast coupling.

Features and Benefits

• Strong absorption
• High fluorescence quantum yield
• High thermal and photo-stability

Other Notes

Fluorescence of bioconjugates[1][2]; employed in fluorescence resonance energy transfer, FRET, experiments[3].

Legal Information

This product is for Research use only. In case of intended commercialization, please contact the IP-holder (ATTO-TEC GmbH, Germany) for licensing.

Safety Information

• Storage Class: 11 - Combustible Solids
• wgk: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, type N95 (US)