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56304

Sigma-Aldrich

Atto 565-Streptavidin

BioReagent, suitable for fluorescence

Synonyma:

Streptavidin-Atto 565

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About This Item

MDL number:
MFCD04040676
UNSPSC Code:
12352108
NACRES:
NA.32

product line

BioReagent

manufacturer/tradename

ATTO-TEC GmbH

fluorescence

λex 565 nm; λem 590 nm in 0.1 M phosphate pH 7.0

suitability

corresponds for gel electrophoresis
suitable for fluorescence

storage temp.

−20°C

Application

Atto 565 is a new label with high molecular absorption (120.000) and quantum yield (0.9) as well as sufficient stoke′s shift (excitation maximum 563 nm, emission maximum 592 nm). It does nearly not show cis trans-isomerisation, which limits brightness and reproducibility for many other dyes. Due to an insignificant triplet formation rate it is well suited for single molecule detection applications. Streptavidin conjugation of Atto 565 does not significantly change the labels spectral data regarding excitation and emission maxima. Atto 565-Streptavidin is used to label biotin conjugated molecules.

Analysis Note

free of unconjugated dye

Legal Information

This product is for Research use only. In case of intended commercialization, please contact the IP-holder (ATTO-TEC GmbH, Germany) for licensing.

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

Osvědčení o analýze (COA)

Vyhledejte osvědčení Osvědčení o analýze (COA) zadáním čísla šarže/dávky těchto produktů. Čísla šarže a dávky lze nalézt na štítku produktu za slovy „Lot“ nebo „Batch“.

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Sigma-Aldrich

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The journal of physical chemistry. B, 115(43), 12607-12614 (2011-09-24)
Recently, studies have been reported in which fluorescently labeled redox proteins have been studied with a combination of spectroscopy and electrochemistry. In order to understand the effect of the dye on the protein-electrode interaction, voltammetry and surface analysis have been
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