This product is soluble in water (50 mg/ml), yielding a clear, colorless solution. DTT is also soluble in ethanol, acetone, ethylate, chloroform, and ether. For gel electrophoresis, a more suitable product would be product D9163. Here is the product data sheet for D9163 which describes solution preparation and use for a gel electrophoresis application: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/917/548/d9163pis.pdf
D0632
DL-Dithiothreitol
≥98% (HPLC), ≥99.0% (titration)
Synonyma:
(±)-Dithiothreitol, rac-Dithiothreitol, Dithiothreitol, threo-1,4-Dimercapto-2,3-butanediol, Cleland’s reagent, DTT
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Vybrat velikost
Změnit zobrazení
| Velikost balení | Skladová položka | Dostupnost | Cena |
|---|---|---|---|
| 1 g | Obraťte se na zákaznický servis a vyžádejte si informaci o dostupnosti. | 1 820,00 Kč | |
| 5 g | Obraťte se na zákaznický servis a vyžádejte si informaci o dostupnosti. | 5 350,00 Kč | |
| 10 g | Obraťte se na zákaznický servis a vyžádejte si informaci o dostupnosti. | 10 200,00 Kč | |
| 25 g | Obraťte se na zákaznický servis a vyžádejte si informaci o dostupnosti. | 25 100,00 Kč | |
| 100 g | Obraťte se na zákaznický servis a vyžádejte si informaci o dostupnosti. | 57 600,00 Kč | |
| 1 kg | Obraťte se na zákaznický servis a vyžádejte si informaci o dostupnosti. | 393 800,00 Kč |
O této položce
Lineární vzorec:
HSCH2CH(OH)CH(OH)CH2SH
Číslo CAS:
Molekulová hmotnost:
154.25
UNSPSC Code:
12352201
NACRES:
NA.21
PubChem Substance ID:
EC Number:
222-468-7
Beilstein/REAXYS Number:
1719757
MDL number:
1 820,00 Kč
Obraťte se na zákaznický servis a vyžádejte si informaci o dostupnosti.
Technický servis
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Dovolte nám, abychom vám pomohliQuality Segment
assay
≥98% (HPLC), ≥99.0% (titration)
form
powder
reaction suitability
reagent type: reductant
color
white
mp
41-44 °C (lit.)
solubility
H2O: soluble 50 mg/mL, clear, colorless to very faintly yellow
application(s)
general analytical
storage temp.
2-8°C
SMILES string
O[C@H](CS)[C@H](O)CS
InChI
1S/C4H10O2S2/c5-3(1-7)4(6)2-8/h3-8H,1-2H2/t3-,4-/m1/s1
InChI key
VHJLVAABSRFDPM-QWWZWVQMSA-N
General description
Dithiothreitol (DTT) is a disulfide (SH) reducing agent found in proteins and in the amino acid cysteine. It reduces a disulfide bond by forming a six-membrane ring and initiating a thiol-disulfide interchange. It is almost 7-fold stronger reducing agent than βME, hence efficient in lower concentrations.
Application
An excellent reagent for maintaining SH groups in reduced state; quantitatively reduces disulfides. DTT is effective in sample buffers for reducing protein disulfide bonds prior to SDS-PAGE. DTT can also be used for reducing the disulfide bridge of the cross-linker N,N′-bis(acryloyl)cystamine to break apart the matrix of a polyacrylamide gel. DTT is less pungent and is less toxic than 2-mercaptoethanol. Typically, a seven fold lower concentration of DTT (100 mM) is needed than is used for 2-mercaptoethanol (5% v/v, 700 mM).
DTT has been used:
- as one of the reactants in the reduction and alkylation of αs1-Casein, the major allergen of cow′s milk.[1]
- as a component of medium for the demembranation and reactivation of spermatozoa.[2]
- to maintain stability of the enzyme as thiol effectively protects the active sites of the biocatalyst.[3]
- as a reducing agent to test the specificity of the reaction of N-Ethylmaleimide with sulfhydryl groups.[4]
- in proteomics analysis as in-solution protein digestion for mass spectrometry[5]
- as a buffer component for protein quantification, to prepare wash buffer, lysis buffer, sample buffer, and protein elution buffer[6][4]
Biochem/physiol Actions
Dithiothreitol (DTT) is extensively applied in chemical peptide synthesis and biochemical preparations of thiol proteins. It is also used in protein chemistry studies, such as protein folding and enzyme activity. It also acts as an enzyme stabilizer to stop DNA modification. DTT specifically mediates the thiol-disulfide interchange reaction to entirely reduce the intra- or inter-molecular disulfide bonds in biomolecules. This reaction results in the formation of thiols and the cyclic disulfide of DTT.[7]
Features and Benefits
- High-quality DTT (HPLC≥98%), (titration≥99.0%)
- Suitable for electrophoresis, proteomics analysis
Other Notes
For additional information on our range of Biochemicals, please complete this form.
1 of 1
Tato položka | |||
|---|---|---|---|
| assay ≥98% (HPLC), ≥99.0% (titration) | assay ≥99.5% (RT) | assay ≥99.0% (titration) | assay ≥99% |
| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level 200 |
| application(s) general analytical | application(s) - | application(s) - | application(s) - |
| form powder | form powder | form powder | form powder |
| storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C |
| mp 41-44 °C (lit.) | mp 41-44 °C (lit.) | mp 41-44 °C (lit.) | mp 40-44 °C, 41-44 °C (lit.) |
Still not finding the right product?
Explore all of our products under DL-Dithiothreitol
signalword
Danger
hcodes
Hazard Classifications
Acute Tox. 4 Oral - Eye Dam. 1 - Skin Irrit. 2
Skladovací třída
11 - Combustible Solids
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
dust mask type N95 (US), Eyeshields, Faceshields, Gloves
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Sortimentní položky
Compare columns in resolving medium-sized antibody fragments after digestion with DTT or IdeS using Reversed-Phase Chromatography for analysis.
Související obsah
Florian Füssl et al.
mAbs, 11(1), 116-128 (2018-10-09)
Charge variant analysis is a widely used tool to monitor changes in product quality during the manufacturing process of monoclonal antibodies (mAbs). Although it is a powerful technique for revealing mAb heterogeneity, an unexpected outcome, for example the appearance of
O Linhart et al.
Reproduction (Cambridge, England), 124(5), 713-719 (2002-11-06)
This study investigated the effects of different environmental conditions on the motility parameters of paddlefish (Polyodon spathula) spermatozoa. Paddlefish spermatozoa demonstrated the following characteristics: (i) all spermatozoa were motile 10 s after activation with a velocity of 130-160 microm s(-1);
Daniel C Marcus et al.
Auditory neuroscience, 1, 101-109 (1994-01-01)
Vestibular dark cell epithelium was isolated from the semicircular canal of gerbils to test the proposal that the sulfhydryl alkylating agent N-ethylmaleimide (NEM) inhibits K(+) secretion by this tissue and does so by reacting with a site in or near
Globální číslo obchodní položky
| Skladová položka | GTIN |
|---|---|
| D0632-10G | 04061835506194 |
| D0632-1KG | 04061833557938 |
| D0632-100G | 04061835518067 |
| D0632-1G | 04061835506200 |
| D0632-25G | 04061833557945 |
| D0632-5G | 04061835515943 |
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What solvent should you use to dissolve DTT in it? Or can you just use H2O? It is for gel electrophoresis on human serum. D0632 DL-Dithiothreitol
1 answer-
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Ist eine 1000 mM DTT Lösung in Wasser stabil, wenn man sie einfriert (-20°C)? Ist DTT (5mM D-0632) in einem Pipes-Puffer pH. 7,1 bei 15°C stabil, wenn man die Lösung bei -20°C einfriert?
1 answer-
Stability and storage data for the reconstituted material is unavailable. Generally, a solution of DTT in Hepes buffer, pH 7.75 is stable for one week at 2 to 8 °C if the container is tightly sealed and the solution is protected from atmospheric oxygen by argon or nitrogen. It is recommended to store the solution for one week at 2 to 8 °C as above or to prepare the solution fresh.
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How can I determine the shelf life / expiration / retest date of this product?
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If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/418/501/product-dating-information-06-25-mk.pdfHelpful?
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How is shipping temperature determined? And how is it related to the product storage temperature?
1 answer-
Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf
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How long can the powder be left out a room temperature?
1 answer-
The long term storage recommendation for this product is refrigerated at 2-8°C, but is shipped at ambient temperature. Refrigerated products that ship at ambient temperature are stable for up to 2 weeks to allow for transit time. Items left at room temperature for short periods will not be adversely effected.
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What is the shelf life of this product once the stock solution is made up please?
1 answer-
A solution of DTT in Hepes buffer, pH 7.75 is stable for one week at 2 to 8 °C if the container is tightly sealed and the solution is protected from atmospheric oxygen by argon or nitrogen.
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