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A8456

4-(2-Aminoethyl)benzenesulfonyl fluoride hydrochloride

≥97.0% (HPLC)

Synonyma:

AEBSF

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Vybrat velikost

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Vám/Skladová položkaDostupnostCena
25 mg
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1 600,00 Kč
100 mg
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5 090,00 Kč
500 mg
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17 200,00 Kč

O této položce

Empirický vzorec (Hillův zápis):
C8H10FNO2S · HCl
Číslo CAS:
Molekulová hmotnost:
239.69
UNSPSC Code:
12352202
NACRES:
NA.77
PubChem Substance ID:
MDL number:
Beilstein/REAXYS Number:
7604627

1 600,00 Kč


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biological source

synthetic (organic)

Quality Segment

assay

≥97.0% (HPLC)

form

powder

solubility

H2O: 50 mg/mL

storage temp.

−20°C

SMILES string

FS(C1=CC=C(CCN)C=C1)(=O)=O.[H]Cl

InChI

1S/C8H10FNO2S.ClH/c9-13(11,12)8-3-1-7(2-4-8)5-6-10;/h1-4H,5-6,10H2;1H

InChI key

WRDABNWSWOHGMS-UHFFFAOYSA-N

General description

stable for up to six months if stored refrigerated at a pH of less than 7. If a pH of greater than 7 is required, pH adjustment should be made just prior to use.

Application

4-(2-Aminoethyl)benzenesulfonyl fluoride hydrochloride (AEBSF) has been used:
  • as a protease inhibitor in plasma samples to prevent acylated ghrelin (AG) degradation
  • as a snake venom serine proteinase (SVSP) inhibitor in SVSP inhibition or AEBSF assay to test the involvement of SVSPs on the fibrinogen-clotting activity
  • as a component in NP-40 lysis buffer to resuspend cell pellets for preparing cell lysates

Recommended concentrations for use are 0.1-1 mM.

Biochem/physiol Actions

4-(2-Aminoethyl)benzenesulfonyl fluoride hydrochloride (AEBSF) is an irreversible serine protease inhibitor. Inhibition constants are similar to those of phenylmethanesulfonyl fluoride (PMSF) and diisopropylfluorophosphate (DFP). AEBSF has been shown to inhibit trypsin, chymotrypsin, plasmin kallikrein, and thrombin. As an alternative to PMSF and DFP, AEBSF offers lower toxicity, improved solubility in water, and improved stability in aqueous solutions. AEBSF can be used in cell culture in concentrations of up to 0.25 mM.
AEBSF can inhibit acetylcholinesterase (AChE).

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Tato položka
1.24839101500SBR00015
biological source

synthetic (organic)

biological source

-

biological source

-

biological source

-

assay

≥97.0% (HPLC)

assay

≥98% (HPLC)

assay

>97% (HPLC)

assay

≥97%

Quality Level

300

Quality Level

100

Quality Level

100

Quality Level

200

form

powder

form

solid

form

solid

form

liquid

solubility

H2O: 50 mg/mL

solubility

10 g/L

solubility

water: 25 mg/mL

solubility

-

storage temp.

−20°C

storage temp.

2-8°C

storage temp.

−20°C

storage temp.

−20°C


pictograms

Corrosion

signalword

Danger

hcodes

Hazard Classifications

Eye Dam. 1 - Skin Corr. 1A

Skladovací třída

8A - Combustible corrosive hazardous materials

flash_point_f

Not applicable

flash_point_c

Not applicable



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Osvědčení o analýze (COA)

Lot/Batch Number

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Questions

1–6 of 6 Questions  
  1. How can I determine the shelf life / expiration / retest date of this product?

    1 answer
    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/418/501/product-dating-information-06-25-mk.pdf

      Helpful?

  2. How is shipping temperature determined? And how is it related to the product storage temperature?

    1 answer
    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

      Helpful?

  3. What is the recommended working concentration for Product A8456, 4-(2-Aminoethyl)benzenesulfonyl fluoride hydrochloride?

    1 answer
    1. Our recommended working concentration range is 0.1 mM to 1 mM; the optimal concentration will depend on the application.

      Helpful?

  4. In what solvents is Product A8456, 4-(2-Aminoethyl)benzenesulfonyl fluoride hydrochloride, soluble?

    1 answer
    1. It is highly soluble in water and in DMSO, at 50 mg/mL (approximately 200 mM), yielding a clear colorless solution. AEBSF may be used in cell culture medium at concentrations up to 0.25 mM.

      Helpful?

  5. How stable is Product A8456, 4-(2-Aminoethyl)benzenesulfonyl fluoride hydrochloride, in solution?

    1 answer
    1. Solutions in water are slightly acidic and retain inhibitory activity for up to six months when stored refrigerated. Solutions at pHs above 7 are less stable.  Stock solutions should be stored at a pH less than 7. If a final pH of greater than 7 is required, the pH adjustment should be done shortly before use.

      Helpful?

  6. What is the Department of Transportation shipping information for this product?

    1 answer
    1. Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.

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