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EAGLETMMRO

Roche

EagleTaq Universal Master Mix (ROX)

sufficient for 100 reactions, sufficient for 500 reactions, sufficient for 5000 reactions, suitable for qPCR, suitable for RT-qPCR

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About This Item

UNSPSC Code:
41106300
NACRES:
NA.55

usage

sufficient for 100 reactions
 sufficient for 500 reactions
 sufficient for 5000 reactions

Quality Level

100

feature

dNTPs included
hotstart

manufacturer/tradename

Roche

packaging

pkg of 100 x 20 μL reactions (07260288190)
pkg of 500 x 20 μL reactions (07260296190)
pkg of 5000 x 20 μL reactions (07249926190)

technique(s)

RT-qPCR: suitable
qPCR: suitable

input

purified DNA

detection method

probe-based

General description

2× concentrated, ready-to-use hot start master mix for qPCR and qRT-PCR using the hydrolysis probe detection format on real-time PCR instruments.

The EagleTaq Universal Master Mix (ROX) is a ready-to-use, 2× concentrated PCR master mix that contains all the reagents (except primers, probes, and template) needed for performing quantitative, real-time PCR hydrolysis probe reactions. It contains a special ROX reference dye which makes it suitable for all real-time instruments on which a ROX reference dye is needed for quantitative analysis.
The EagleTaq Universal Master Mix (ROX) contains dUTP so that it may be used with Uracil-DNA Glycosylase (UNG) to prevent false positives arising from carryover contamination.
The hot start properties allow reaction setup at ambient temperature.
With this robust reagent, any PCR protocol optimization is minimized
The EagleTaq Universal Master Mix (ROX) is a ready-to-use, 2× concentrated polymerase chain reaction (PCR) master mix that contains all the reagents (except primers, probes, and template) needed for performing quantitative, real-time PCR (qPCR) and quantitative reverse transcription PCR (qRT-PCR) hydrolysis probe reactions.

Application

EagleTaq Universal Master Mix (ROX) has been used in quantitative, real-time polymerase chain reaction (qPCR ) and quantitative reverse transcription PCR (qRT-PCR).

Features and Benefits

  • The EagleTaq Universal Master Mix (ROX) contains a special ROX reference dye which makes it suitable for all real-time instruments on which a ROX reference dye is needed for quantitative analysis.
  • It contains dUTP so that it may be used with Uracil-DNA Glycosylase (UNG) to prevent false positives arising from carryover contamination.
  • The hot start properties allow reaction setup at ambient temperature.
  • With this robust reagent, any PCR protocol optimization is minimized.

Components

Contains EagleTaq DNA Polymerase and a reaction buffer with dATP, dCTP, dGTP, dUTP, MgCl2, and ROX.

Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 1

flash_point_f

does not flash

flash_point_c

does not flash

Osvědčení o analýze (COA)

Vyhledejte osvědčení Osvědčení o analýze (COA) zadáním čísla šarže/dávky těchto produktů. Čísla šarže a dávky lze nalézt na štítku produktu za slovy „Lot“ nebo „Batch“.

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Sortimentní položky

PCR Master Mix

PCR master mix simplifies PCR/RT-PCR with components like DNA polymerase, dNTPs, MgCl2, and buffer, available commercially or DIY.

Související obsah

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Polymerázová řetězová reakce (PCR) je technika pro amplifikaci molekul nukleových kyselin a běžně se používá v mnoha aplikacích, včetně RT-PCR, PCR s horkým startem, PCR s koncovým bodem a dalších.

PCR Applications

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