10988537001

7-Deaza-2′-deoxy-guanosine-5′-triphosphate

95% (7-Deaza-dGTP, HPLC), ≤4% (7-Deaza-dGDP, HPLC), solution, 10 mM

• Synonyma: 7-Deaza-2′-deoxyguanosine 5′-triphosphate lithium salt, −N⁷-dGTP, 7-Deaza-dGTP
• Empirický vzorec (Hillův zápis): C₁₁H₁₇N₄O₁₃P₃
• Číslo CAS: 101515-08-6
• Molekulová hmotnost: 506.19
• MDL number: MFCD00171614
• UNSPSC Code: 41116100
• PubChem Substance ID: 329749263

Vlastnosti

• Quality Level: 100
• form: solution
• composition: 7-Deaza-dGDP, ≤4% HPLC ; 7-Deaza-dGTP, 95% HPLC
• packaging: pkg of 200 μL (10 mM, 2 μmol)
• manufacturer/tradename: Roche
• concentration: 10 mM
• storage temp.: −20°C
• SMILES string: NC1=NC(=O)c2ccn(C3CC(O)C(COP(O)(=O)OP(O)(=O)OP(O)(O)=O)O3)c2N1
• InChI: 1S/C11H17N4O13P3/c12-11-13-9-5(10(17)14-11)1-2-15(9)8-3-6(16)7(26-8)4-25-30(21,22)28-31(23,24)27-29(18,19)20/h1-2,6-8,16H,3-4H2,(H,21,22)(H,23,24)(H2,18,19,20)(H3,12,13,14,17)
• InChI key: DLLXAZJTLIUPAI-UHFFFAOYSA-N

Popis

General description

7-deaza-2′-deoxy-guanosine-5′-triphosphate is a nucleotide analog that is a candidate substrate for processive telomerase. The use of this analog improves PCR (polymerase chain reaction) yields. This compound also makes sequencing of GC rich sequences easier, especially if template amount is low and/or DNA quality is not good.

The product contains 7-Deaza-2′-deoxy-guanosine-5′-triphosphate as a 10 mM solution of the lithium salt.

Application

7-Deaza-dGTP is a substrate for most DNA polymerases, including Taq DNA polymerase.
7-Deaza-dGTP is used in the dideoxy-chain termination sequencing methods, in place of dGTP to overcome compression problems in gel electrophoresis when sequencing GC-rich stretches of DNA.
Partial substitution of 7-Deaza-dGTP for dGTP in PCRs can improve the product yield when the template is GC-rich (i.e., has extensive secondary structures).

Preparation Note

Working solution: Dilute 7-deaza-dGTP into a solution containing 50 mM Tris, pH 7.0, and use at exactly the same concentration as you would use dGTP.

Analysis Note

Absorption: 25 A₂₆₀ units of product correspond to 1 mg.

Absorbance determination: A₂₅₀/A₂₆₀, A₂₈₀/A₂₆₀, A₂₉₀/A₂₆₀

Other Notes

When PCR products contain 7-Deaza dGTP, they do not stain well in ethidium bromide, presumably because stacking of adjacent bases in the products is diminished.
PCR amplification efficiency is generally not affected by 7-Deaza-dGTP.
For life science research only. Not for use in diagnostic procedures.

Bezpečnostní informace

• Storage Class: 12 - Non Combustible Liquids
• wgk_germany: WGK 1
• flash_point_f: No data available
• flash_point_c: No data available