ABN185

Anti-alpha B-crystallin Antibody

from rabbit, purified by affinity chromatography

• Synonym(s): Alpha-crystallin B chain, Alpha(B)-crystallin, Heat shock protein beta-5, Renal carcinoma antigen NY-REN-27, Rosenthal fiber component
• UNSPSC Code: 12352203
• NACRES: NA.41
• eCl@ss: 32160702
• Conjugate: unconjugated
• Clone: polyclonal
• Application: IHC, WB
• Citations: 8

Properties

• biological source: rabbit
• Quality Segment: 100
• conjugate: unconjugated
• antibody form: affinity isolated antibody
• antibody product type: primary antibodies
• clone: polyclonal
• purified by: affinity chromatography
• species reactivity: bovine, human, rat
• species reactivity (predicted by homology): horse (based on 100% sequence homology), rhesus macaque (based on 100% sequence homology), ox (based on 100% sequence homology), mouse (based on 100% sequence homology)
• technique(s): immunohistochemistry: suitable (paraffin), western blot: suitable
• NCBI accession no.: NP_001876
• UniProt accession no.: P02511
• shipped in: wet ice
• target post-translational modification: unmodified
• Gene Information: bovine ... Cryab(281719) ; human ... CRYAB(1410) ; mouse ... Cryab(12955) ; rat ... Cryab(25420)

Description

General description

Alpha B-crystallin is a major structural protein in the vertebrate eye lens, but is also found in cardiac and skeletal muscles, kidney, and brain. Alpha B-crystallin is also found to be a component of Rosenthal fibers and a consistent tissue marker of epileptic foci. It occurs as large aggregates of up to 106 Da, composed of two proteins, αA- and αB-crystallin. The αA gene encodes a polypeptide of 173 residues, while αB-crystallin gene encodes a 175 residue polypeptide. αB-crystallin, like the small heat shock proteins (sHsp′s), is induced by heat or osmotic shock and is upregulated by stress. α-crystallin and the sHsp′s protect other proteins from heat-induced aggregation. The two other crystallin families, β and ɣ, are homologous to each other but not to the α-family or the sHsp′s.

~20 kDa observed. Uncharacterized bands at ~37 and 43 kDa may be observed in some cell lyates.

Immunogen

Epitope: N-terminus

KLH-conjugated linear peptide corresponding to the N-terminus human alpha B-crystallin.

Application

Immunohistochemistry Analysis: A 1:1,000 dilution from a representative lot detected Alpha-crystallin B chain in rat lens fiber tissue. A 1:2,000 dilution from a representative lot detected Alpha-crystallin B chain in strong rat lens fiber tissue (data not provided). A 1:1,000 dilution from a representative lot detected Alpha-crystallin B chain in strong muscle fiber tissue (data also not provided). It is recommended to use a 1:2,000 dilution only for strong rat lens fiber tissue.
Western Blot analysis: 0.05 µg/mL of this antibody detected Alpha-crystallin B chain in 10 µg of human kidney and calf kidney tissue lysate.

Research Category
Neuroscience

Research Sub Category
Developmental Neuroscience

This Anti-alpha B-crystallin Antibody is validated for use in WB, IH(P) for the detection of alpha B-crystallin.

Biochem/physiol Actions

This antibody recognizes the N-terminus of alpha B-crystallin.

Physical form

Affinity purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Preparation Note

Stable for 1 year at 2-8°C from date of receipt.

Analysis Note

Control
Bovine calf lens, human kidney and calf kidney tissue lysate

Evaluted by Western Blot in bovine calf lens tissue lysate.

Western Blot analysis: 0.05 µg/mL of this antibody detected Alpha-crystallin B chain in 10 µg of bovine calf lens tissue lysate.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 12 - Non Combustible Liquids
• wgk: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable