AB1936

Anti-Integrin α2 Antibody

serum, Chemicon^®

• Synonym(s): CD49b
• UNSPSC Code: 12352203
• NACRES: NA.41
• eCl@ss: 32160702
• Conjugate: unconjugated
• Clone: polyclonal
• Application: ELISA, IF, IHC, IP, RIA, WB
• Citations: 50

Properties

• biological source: rabbit
• Quality Segment: 100
• conjugate: unconjugated
• antibody form: serum
• antibody product type: primary antibodies
• clone: polyclonal
• species reactivity: chicken, rat, hamster, mouse, human
• manufacturer/tradename: Chemicon^®
• technique(s): ELISA: suitable, immunofluorescence: suitable, immunohistochemistry: suitable, immunoprecipitation (IP): suitable, radioimmunoassay: suitable, western blot: suitable
• NCBI accession no.: NM_002203.3
• UniProt accession no.: P17301
• shipped in: wet ice
• target post-translational modification: unmodified
• Gene Information: human ... ITGA2(3673)

Description

General description

160kDa

Immunogen

Synthetic peptide derived from the COOH terminal sequence (cytoplasmic domain) of the human alpha2 integrin subunit {KYEKMTKNPDEODETTELSS}

Application

Anti-Integrin α2 Antibody detects level of Integrin α2 & has been published & validated for use in ELISA, IF, IH, IP, RIA & WB.

Immunofluorescence: 1:500 (suggested for use on acetone-fixed tissue only)

ELISA/RIA: 1:1000

Western blot: 1:1000 The antibody has been used to report alpha2 expression in HUVECs during tubule-like structure formation in collagen (Deroanne, 1996). Recognizes ~160kDa alpha 2 subunit

Immunoprecipitation: 5 μL precipitates alpha2/beta1 from 5 x 106 cells.

Immunohistochemistry: 1:500

Optimal working dilutions must be determined by end user.

Research Category
Cell Structure

Research Sub Category
Integrins

Biochem/physiol Actions

Human alpha2 integrin subunit. Specificity determined by immunoprecipitation from a detergent extract of 35S methionine labeled endothelial cells. The epitope recognized is on the cytoplasmic domain of the protein and is thus intracellular. Shows no cross-reactivity to alpha1, alpha3, alpha4, alpha5, alpha6, or alphaV subunits.

Physical form

Neat rabbit antiSerum. Liquid with 0.05% sodium azide

Unpurified

Preparation Note

Maintain for 1 year at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Analysis Note

Control
Skin (basement membrane), High grade ovarian tumors

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 10 - Combustible liquids
• wgk: WGK 1