454850-M

MAP Kinase 2, Mouse, Recombinant, E. coli

• Synonyma: ERK2, MAPK2, p42 ^MAPK
• UNSPSC Code: 12352202
• NACRES: NA.54
• Specific activity: ≥5000 units/mL
• Assay: ≥90% (SDS-PAGE)
• Recombinant: expressed in E. coli

Vlastnosti

• recombinant: expressed in E. coli
• Quality Segment: 100
• assay: ≥90% (SDS-PAGE)
• form: liquid
• specific activity: ≥5000 units/mL
• manufacturer/tradename: Calbiochem^®
• storage condition: OK to freeze, avoid repeated freeze/thaw cycles
• foreign activity: MEK, phosphatase, protease, none detected
• storage temp.: −70°C

Popis

General description

Recombinant, mouse MAP Kinase 2 expressed in E. coli. A serine/threonine protein kinase that acts at the end of a protein kinase cascade linking signals from cell surface receptor tyrosine kinases to cytoplasmic and nuclear events. This protein was co-expressed with MEK and is phosphorylated and active.

Physical form

In 100 mM NaCl, 50 mM HEPES, 1 mM DTT, 100 µM Na₂-EDTA, 50% glycerol, 0.01% Brij^® 35 detergent, pH 7.5.

Preparation Note

Following initial thaw, aliquot and freeze (-70°C).

Other Notes

Fukunaga, K., and Miyamoto, E. 1998. Mol. Neurobiol. 16, 79.
Wu, J., et al. 1993. Mol. Cell. Biol.13, 4539.

One unit is defined as the amount of enzyme that will catalyze the transfer of 1.0 pmol phosphate to myelin basic protein per min at 30°, pH 7.5.

Legal Information

Brij is a registered trademark of Croda International PLC

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Toxicity: Standard Handling (A)

Bezpečnostní informace

• Skladovací třída: 10 - Combustible liquids
• wgk: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable