14-440-M

PKA Protein, Recombinant, 10 µg

Recombinant human full length PKA, catalytic subunit type alpha, untagged, for use in Kinase Assays.

• UNSPSC Code: 12352202
• NACRES: NA.41
• eCl@ss: 32160405
• Form: liquid
• Biological source: human
• Recombinant: expressed in E. coli, expressed in BL21 DE3 cells
• Mol wt: M_w 40.7 kDa

Properties

• biological source: human
• Quality Segment: 100
• recombinant: expressed in E. coli, expressed in BL21 DE3 cells
• product line: Upstate^®
• form: liquid
• specific activity: (For Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.), 8580 U/mg
• shelf life: 1 yr
• mol wt: M_w 40.7 kDa
• purified by: chromatography
• species reactivity: human
• manufacturer/tradename: Upstate^®
• technique(s): activity assay: suitable (kinase)
• solubility: water: soluble
• accession no.: (FUNCTION: SwissProt: P17612 # Phosphorylates a large number of substrates in the cytoplasm and the nucleus. ), NM_002730.3
• NCBI accession no.: NM_002730.3, NM_207518.1
• UniProt accession no.: P17612
• storage temp.: −70°C
• Gene Information: human ... PRKACA(46909581)

Description

General description

Research area: Cell Signaling

Product Source: Expressed in E.coli BL21 (DE3) pLysS cells Recombinant human full length PKA, catalytic subunit type alpha, untagged Protein kinase A (PKA) is a serine protein kinase and heterotetrameric holoenzyme that targets serine and threonine residues.

PKA

Recombinant human full length PKA, catalytic subunit type alpha, untagged

Biochem/physiol Actions

Protein Target: PKA; Target Sub-Family: AGC. Protein kinases are vital for integrating signaling networks within eukaryotic cells. In Hedgehog-responsive cells, PKA is crucial for regulating proliferation and fate determination by influencing Hedgehog signaling transduction. PKA has numerous targets that are linked to adhesion or migration.

Packaging

Also available in 250 µg size --call for pricing and availability and reference catalog number 14-440M when ordering the 250 µg size.

Also available in 10 μg size and in bulk

Physical form

Phosphocellulose P11 chromatography

Preparation Note

1 year at -70°C

Analysis Note

routinely evaluated by phosphorylation of Kemptide substrate in vitro

Other Notes

For Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.

Replaces: 14-114

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 10 - Combustible liquids
• wgk: WGK 1