14-306-M

GSK3β Protein, active, 10 µg

Active, N-terminal His6-tagged recombinant human GSK3β with an H350L mutation, for use in Kinase Assays.

• UNSPSC Code: 12352202
• NACRES: NA.26
• eCl@ss: 32160405
• Form: liquid
• Biological source: human
• Recombinant: expressed in baculovirus infected Sf21 cells
• Mol wt: M_w 51 kDa

Properties

• biological source: human
• Quality Segment: 100
• recombinant: expressed in baculovirus infected Sf21 cells
• form: liquid
• mol wt: M_w 51 kDa
• composition: mercaptoethanol, 0.1-1% , saccharose, 5-10%
• manufacturer/tradename: Upstate^®
• storage condition: (Tightly closed)
• technique(s): activity assay: suitable (kinase)
• solubility: water: soluble
• NCBI accession no.: NM_002093.2
• UniProt accession no.: P49841
• Gene Information: human ... GSK3B(2932)

Description

General description

Glycogen synthase kinase 3β (GSK3β) is a serine-threonine kinase that is present in most eukaryotes. The GSK3β gene is located on the human chromosome at 3q13.3.[1] GSK3β is made up of an N-terminal β-sheet domain that is coupled to the C-terminal α-helical domain.

Research area: Cell Signaling

Biochem/physiol Actions

Glycogen synthase kinase 3β (GSK3β) regulates glycogen metabolism[2] via an insulin-dependent pathway.[1] It participates in many signaling pathways including cellular processes such as cell cycle, cell proliferation, and inflammation. GSK3β mediated-negative regulation of insulin signaling pathway leads to type 2 diabetes.[2] GSK3β functions as a tumor suppressor that represses neoplastic transformation in cells. It is an essential regulator of the Wnt/β-catenin signaling pathway. Abnormal regulation of GSK3β is associated with cardiovascular disease, bipolar disorder, and neurodegenerative diseases.[1]

Packaging

Also available in 250μg size (2x125μg)--call for pricing and availability and reference catalog number 14-306M when ordering the 250μg size.

Analysis Note

Routinely evaluated by phosphorylation of Phospho-Glycogen Synthase Peptide-2

Other Notes

For Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• pictograms: GHS07
• signalword: Warning
• hcodes: H317
• pcodes: P261 - P272 - P280 - P302 + P352 - P333 + P313 - P362 + P364
• Hazard Classifications: Skin Sens. 1
• Storage Class: 12 - Non Combustible Liquids
• wgk: WGK 2
• flash_point_f: Not applicable
• flash_point_c: Not applicable