Přejít k obsahu
Merck
Všechny fotografie(2)

Key Documents

810184P

Avanti

TopFluor™ PI(4,5)P2

Avanti Research™ - A Croda Brand 810184P, powder

Synonyma:

1-oleoyl-2-{6-[4-(dipyrrometheneboron difluoride)butanoyl]amino}hexanoyl-sn-glycero-3-phosphoinositol-4,5-bisphosphate (ammonium salt)

Přihlásitk zobrazení cen stanovených pro organizaci a smluvních cen
Všechny fotografie(2)

About This Item

Empirický vzorec (Hillův zápis):
C50H78BF2N3O20P3
Číslo CAS:
1246355-57-6
Molekulová hmotnost:
1182.89
UNSPSC Code:
12352211
NACRES:
NA.25

assay

>99% (TLC)

form

powder

packaging

pkg of 1 × 50 μg (with stopper and crimp cap (810184P-50ug))

manufacturer/tradename

Avanti Research™ - A Croda Brand 810184P

shipped in

dry ice

storage temp.

−20°C

General description

Although PI(4,5)P2 is a minor component of cell membranes, it plays a critical role as a substrate for a number of important signaling proteins. PI(4,5)P2 is an intermediate in the IP3/DAG pathway where it is hydrolyzed by phospholipase C to liberate the second messengers, inositol 1,4,5-triphosphate (IP3) and diacylglycerol (DAG). PI(4,5)P2 is also a substrate for PI 3-kinase where it is phosphorylated to PI(3,4,5)P3, an activator of downstream signaling components such as the protein kinase AKT.

Application

TopFluor™ PI(4,5)P2 or 1-oleoyl-2-{6-[4-(dipyrrometheneboron difluoride)butanoyl]amino}hexanoyl-sn-glycero-3-phosphoinositol-4,5-bisphosphate (ammonium salt) has been used as a fluorescent lipid in living cells to analyse the cellular localization of phosphatidylinositol (PI) and in the preparation of giant unilamellar vesicles (GUVs) for the ezrin-membrane tethering assays.

Packaging

2 mL Amber Serum Vial with Stopper and Crimp Cap (810184P-50ug)

Legal Information

Avanti Research is a trademark of Avanti Polar Lipids, LLC
TopFluor is a trademark of Avanti Polar Lipids, LLC

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

No data available

flash_point_c

No data available

Osvědčení o analýze (COA)

Vyhledejte osvědčení Osvědčení o analýze (COA) zadáním čísla šarže/dávky těchto produktů. Čísla šarže a dávky lze nalézt na štítku produktu za slovy „Lot“ nebo „Batch“.

Již tento produkt vlastníte?

Dokumenty související s produkty, které jste v minulosti zakoupili, byly za účelem usnadnění shromážděny ve vaší Knihovně dokumentů.

Navštívit knihovnu dokumentů

Ezrin enrichment on curved membranes requires a specific conformation or interaction with a curvature-sensitive partner
Tsai FC, et al.
eLife, 7, e37262-e37262 (2018)
Feng-Ching Tsai et al.
eLife, 7 (2018-09-21)
One challenge in cell biology is to decipher the biophysical mechanisms governing protein enrichment on curved membranes and the resulting membrane deformation. The ERM protein ezrin is abundant and associated with cellular membranes that are flat, positively or negatively curved.
Xiaojun Shi et al.
Langmuir : the ACS journal of surfaces and colloids, 32(7), 1732-1741 (2016-02-02)
Phosphatidylinositol phosphate (PIP) lipids are critical to many cell signaling pathways, in part by acting as molecular beacons that recruit peripheral membrane proteins to specific locations within the plasma membrane. Understanding the biophysics of PIP-protein interactions is critical to developing
Probing the subcellular distribution of phosphatidylinositol reveals a surprising lack at the plasma membrane.
James PZ, et al.
The Journal of Cell Biology, 219(3), e201906127-e201906127 (2020)
Maria J Sarmento et al.
Langmuir : the ACS journal of surfaces and colloids, 33(43), 12463-12477 (2017-09-30)
Although the evidence for the presence of functionally important nanosized phosphorylated phosphoinositide (PIP)-rich domains within cellular membranes has accumulated, very limited information is available regarding the structural determinants for compartmentalization of these phospholipids. Here, we used a combination of fluorescence
Zobrazit všechny související dokumenty

Náš tým vědeckých pracovníků má zkušenosti ve všech oblastech výzkumu, včetně přírodních věd, materiálových věd, chemické syntézy, chromatografie, analytiky a mnoha dalších..

Obraťte se na technický servis.