CFPS700
Next Generation Cell Free Protein Expression Kit (Wheat Germ)
An Eukaryotic Cell Free Protein Expression system (CFPE/CFPS) with High Yield and Excellent Scalability, 50 reactions
Synonyma:
cell free protein synthesis
About This Item
Doporučené produkty
technique(s)
protein expression: suitable
shipped in
dry ice
storage temp.
−70°C
General description
Features and Benefits
Next Generation Cell Free Protein Expression Kit (Wheat Germ) (part #: CFPS700) a next generation product protocol to ensure even higher stability, yield and more flexibility for a larger variety of target proteins, such as kinases, membrane proteins transcription factors, antibodies, photoreceptors etc., Our wheat germ extract contains various machines, micelle and organelles. As a result, even membrane protein can be soluble without adding any liposome and disulfide bonds are induced. By using this kit, a protein of interest can be expressed in a day starting from cDNA. There is no need for cloning or cell culture.
- Great Time and cost efficiency, easy to use (batch model)
- Excellent yield: 5.5mL sample scale per kit brings 0.5 mg to 1mg proteins to you in a day at low cost
- No cloning or subcloning necessary for full length or truncation
- Animal- and serum-free
- Of eukaryotic origin
- High solubility due to native conformation and eukaryotic source
- Greater stability
- Suitable for protein labeling and the expression of difficult-to-express proteins like membrane proteins and multiple protein complexes
Components
This product is comprised of the following:
- 1x 25 mM NTP Mix
- 1x T7 RNA Polymerase
- 10x Transcription Buffer
- 1x 100 mM DTT
- 1x Wheat Germ Extract
- 1x Amino Acid Mix
Application
- Expression screening
- High throughput in vitro production of recombinant proteins
- Ribosome Display
- Protein microarrays
- Structural Genomics, NMR (Isotope labeling) or incorporating unnatural amino acid and crystallography
See Instruction for the Primer Design Tool for the 1st PCR
See CFPS700 Primer List (left-click and download the Excel file)
Storage and Stability
Store the components at the designated Temperatures once received.
10x Transcription Buffer: -20 °C
25 mM NTP Mix: -20 °C
T7 RNA Polymerase: -20 °C
100 mM DTT: -20 °C
Wheat Germ Extract: -70 °C
Amino Acid Mix: -70 °C
signalword
Danger
hcodes
Hazard Classifications
Eye Dam. 1 - Skin Irrit. 2
Storage Class
10 - Combustible liquids
flash_point_f
Not applicable
flash_point_c
Not applicable
Osvědčení o analýze (COA)
Vyhledejte osvědčení Osvědčení o analýze (COA) zadáním čísla šarže/dávky těchto produktů. Čísla šarže a dávky lze nalézt na štítku produktu za slovy „Lot“ nebo „Batch“.
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Sortimentní položky
Cell-free protein synthesis rapidly produces recombinant proteins without cells, utilizing translation machinery from different cell types for efficiency.
Protokoly
Next Generation Cell Free Protein Expression Kit (Wheat Germ) (CFPS700) PROTOCOL
Náš tým vědeckých pracovníků má zkušenosti ve všech oblastech výzkumu, včetně přírodních věd, materiálových věd, chemické syntézy, chromatografie, analytiky a mnoha dalších..
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