"Cluster-ion" bombardment of biological macromolecules: Morphological evidence for disassembly of single tobacco mosaic virus particles.

"Cluster-ion" bombardment of biological macromolecules: Morphological evidence for disassembly of single tobacco mosaic virus particles.

Proceedings of the National Academy of Sciences of the United States of America (1987-01-01)

M C Ledbetter, R J Beuhler, L Friedman

ABSTRACT

Cluster ions containing 50, 100, or 200 water molecules and one proton are accelerated to over 275 kV and impacted on thin carbon films on which tobacco mosaic virus has been dispersed. After bombardment the films are examined with a transmission electron microscope to study induced morphological alterations with respect to the energy transfer processes that take place during and after single cluster-ion impact. Observations on tobacco mosaic virus indicate that a single cluster impact results in the removal of a cylindrical segment of the virus. No fragments from the excised TMV segment were observed on the carbon foil. We speculate that cluster-ion bombardment may become a useful analytical technique for structural studies of large biological macromolecular complexes.

MATERIALS

Product Number

Brand

Product Description

GF58259469

Carbon, foil, 50x50mm, thickness 0.2mm, flexible graphite, 99.8%

GF71541031

Carbon, foil, not light tested, 150x150mm, thickness 0.075mm, flexible graphite, 99.8%

GF46425078

Carbon, foil, 50x50mm, thickness 0.2mm, pyrolytic graphite, 99.99%

GF86694069

Carbon, foil, 50x50mm, thickness 0.25mm, rigid graphite, fine grain size, 99.997%

GF71469939

Carbon, foil, 5x5mm, thickness 2.0mm, hOpg

GF01171763

Carbon, foil, 50x50mm, thickness 0.35mm, flexible graphite, 99.8%

GF78424735

Carbon, foil, 5x10mm, thickness 2.0mm, hOpg

GF64932947

Carbon, foil, 10mm disks, thickness 0.2mm, flexible graphite, 99.8%

GF87162857

Carbon, foil, 50x50mm, thickness 0.5mm, flexible graphite, 99.8%

GF00462912

Carbon, foil, 25x25mm, thickness 0.1mm, pyrolytic graphite, 99.99%

GF83177949

Carbon, foil, 12mm disks, thickness 0.5mm, rigid graphite, fine grain size, 99.95%

GF42290939

Carbon, foil, not light tested, 150x150mm, thickness 0.125mm, flexible graphite, 99.8%

GF05561849

Carbon, foil, not light tested, 100x100mm, thickness 0.075mm, flexible graphite, 99.8%

GF23879009

Carbon, foil, 100x100mm, thickness 0.5mm, flexible graphite, 99.8%

GF46470944

Carbon, foil, 100x100mm, thickness 0.35mm, flexible graphite, 99.8%

GF37715182

Carbon, foil, 100x100mm, thickness 0.5mm, rigid graphite, fine grain size, 99.95%

GF86742120

Carbon, foil, 100x100mm, thickness 0.5mm, rigid graphite, fine grain size, 99.997%

GF23417434

Carbon, foil, 100x100mm, thickness 1.0mm, flexible graphite, 99.8%

GF61313600

Carbon, foil, 100x100mm, thickness 1.0mm, rigid graphite, fine grain size, 99.95%

GF78361285

Carbon, foil, 150x150mm, thickness 0.5mm, flexible graphite, 99.8%

GF31912039

Carbon, foil, 150x150mm, thickness 0.5mm, rigid graphite, fine grain size, 99.95%

GF82985844

Carbon, foil, 150x150mm, thickness 1.0mm, rigid graphite, fine grain size, 99.95%

GF11412470

Carbon, foil, 100x100mm, thickness 5.0mm, rigid graphite, medium grain size, 99.5%

GF26559513

Carbon, foil, 150x150mm, thickness 5.0mm, rigid graphite, medium grain size, 99.5%

GF09288906

Carbon, foil, 15x15mm, thickness 2.0mm, hOpg

GF04191971

Carbon, foil, 150x150mm, thickness 0.2mm, flexible graphite, 99.8%

GF29579787

Carbon, foil, 200x200mm, thickness 1.0mm, rigid graphite, fine grain size, 99.95%

GF41284953

Carbon, foil, 25x25mm, thickness 0.5mm, pyrolytic graphite, 99.99%

GF76337871

Carbon, foil, 500x500mm, thickness 1.0mm, flexible graphite, 99.8%

GF13406633

Carbon, foil, 250x250mm, thickness 0.5mm, flexible graphite, 99.8%