Evaluation of Tegaran Formula ZhenHua cytotoxicity against human cancer cell lines.

Evaluation of Tegaran Formula ZhenHua cytotoxicity against human cancer cell lines.

PloS one (2020-10-22)

Panagiotis Parsonidis, Ioanna Vlachou, Alexandra Mamagkaki, Ioannis Bouris, Vasiliki Daikopoulou, Ioannis Papasotiriou

ABSTRACT

The aim of this study is to evaluate the potential health effects of Tegaran Formula ZhenHua, a nutritional supplement used mainly by cancer patients. Its active ingredients and cytotoxicity was assessed with analytical methods and viability assays, respectively. The analytical methods consisted of dissolution, disintegration, HPLC, LC/MS, GC/MS and NMR. Cytotoxicity was assessed by MTT, SRB, CVE colorimetric viability assays in 0, 24, 48 and 72h time points. The results indicate that Tegaran Formula ZhenHua supplement did not present any cytotoxic effects due to issues related to the capsules' solubility, distribution and identification of the active ingredient.

MATERIALS

Product Number

Brand

Product Description

T6791

Sigma-Aldrich

Trizma^® base, BioXtra, pH 10.5-12.0 (1 M in H₂O), ≥99.9% (titration)

M2128

Sigma-Aldrich

Thiazolyl Blue Tetrazolium Bromide, 98%

G7513

Sigma-Aldrich

L-Glutamine solution, 200 mM, solution, sterile-filtered, BioXtra, suitable for cell culture

D5546

Sigma-Aldrich

Dulbecco′s Modified Eagle′s Medium - low glucose, With 1000 mg/L glucose, and sodium bicarbonate, without L-glutamine, liquid, sterile-filtered, suitable for cell culture

R0883

Sigma-Aldrich

RPMI-1640 Medium, With sodium bicarbonate, without L-glutamine, liquid, sterile-filtered, suitable for cell culture

91228

Sigma-Aldrich

Trichloroacetic acid, BioUltra, ≥99.5% (T)

16587

Supelco

Daidzein, analytical standard

HT901

Sigma-Aldrich

Crystal Violet Solution